A new molecularly imprinted polymer was synthesized with malachite green (MG) as molecular template, methacrylic acid(MAA) as functional monomer, ethylene dimethacrylate (EDMA) as crosslinker, and azobisisobutyronitrile (AIBN) as initiator.Recognition properties of the MG imprinted polymer were studied by equilibrium adsorption and HPLC. The results showed that the imprinted polymer had good affinity and marked selectivity for MG, and can separate MG with its analogue commendably. The new polymer can be used for the enrichment of MG in complex sample, and can work as separation media to separate and detect MG by HPLC.