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目的观察不同代次F基因型SP-A株腮腺炎病毒在恒河猴体内的致病性。方法选取腮腺炎病毒SP-A株在KMB17细胞上传至15、20、30代的活病毒作为实验组,在Vero细胞上传至第2代的病毒作为野毒株对照组,分别接种恒河猴腮腺。观察实验动物体温和临床体征。活体取双侧腮腺,进行组织病理检查,并检测腮腺炎病毒中和抗体。结果对照组动物出现了明显的体温升高,一般状况较差,注射后第5天出现腮腺肿大,组织病理检查表现为淋巴细胞大量浸润。而各实验组动物均未出现腮腺炎症状和明显的组织病理学变化。各组动物接种病毒后均能产生抗腮腺炎病毒的中和抗体,但对照组的中和抗体GMT水平均高于各实验组。结论腮腺炎病毒SP-A株传至第15代次,已无致腮腺炎发病能力,为开发F基因型腮腺炎病毒减毒活疫苗奠定了基础。
Objective To observe the pathogenicity of different generations of F genotype Mumps virus in the rhesus monkeys. Methods Mumps virus strain SP-A was used as the experimental group in the 15th, 20th and 30th generations of live virus in KMB17 cells and the second generation virus in Vero cells as control group of wild-type strain, . Observe the experimental animal body temperature and clinical signs. Live parotid biopsy taken for histopathological examination, and detection of mumps virus neutralizing antibodies. Results The animals in the control group showed obvious increase in body temperature. The general condition was poor. Parotid enlargement occurred on the 5th day after injection and the infiltration of lymphocytes was observed by histopathological examination. Mumps symptoms and obvious histopathological changes were not found in all experimental groups. Neither neutralizing antibody against mumps could be produced in any group after inoculation of virus, but the neutralizing antibody GMT level in the control group was higher than that in each experimental group. Conclusion The mumps virus strain SP-A reached the 15th generation and no mumps pathogens were found, which laid the foundation for the development of live attenuated vaccine against mumps virus.