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本工作采用控制营养液中的钙量和叶片喷钙的方法来提高植株的钙水平,研究了钙对水分胁迫大豆光合作用的效应,结果表明:在水分胁迫下,经高钙处理(14mmol/L Ca~(2+)大豆叶片保持高的光合速率;中钙(5mmol/L Ca~(2+))处理次之;低钙(营养液中不加Ca(NO_3)_2,而以NaNO_3代之)处理一周叶片光合作用速率最低,相对变化最大,在同样程度的水分胁迫下,高钙处理叶片具有较高的CO_2羧化效率,其光合作用由气孔限制向非气孔限制的转变亦较晚,田间喷钙,可以提高受旱大豆叶片的光合速率,高钙处理或喷钙增大了气孔对水分胁迫的反应能力,在水分胁迫下气孔阻力很快上升,抑制了水分过分消耗,而光合作用相对活性提高,高钙处理使细胞质膜和叶绿体膜系结构完整性提高,膜上酶活性增大。
The effect of calcium on photosynthesis of soybean by water stress was studied in this work. The results showed that under the condition of water stress, the effect of high calcium treatment (14 mmol / L Ca2 + soybean leaves maintained high photosynthetic rate, followed by medium calcium (5mmol / L Ca2 +), low Ca2 + (Ca (NO3) 2) and NaNO3 ) Had the lowest photosynthetic rate and the largest relative change in the first week. Under the same water stress, the leaves with high calcium had higher CO 2 carboxylation efficiency, and photosynthesis changed from stomatal limitation to non-stomatal limitation later , Sprinkling calcium in the field could increase the photosynthetic rate of drought-stressed soybean leaves, high-calcium treatment or spraying of calcium increased the ability of stomata to respond to water stress. Under water stress, stomatal resistance increased rapidly and inhibited excessive water consumption, while photosynthesis The relative activity increased, and the high calcium treatment increased the structural integrity of the plasma membrane and chloroplast membrane, and increased the enzyme activity on the membrane.