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目的 :探讨子宫颈癌变过程中 ,耐药标志物热休克蛋白 2 7(heatshockprotein 2 7,HSP2 7)和金属硫蛋白 (metallothioneinMT)的表达特点 ,为指导子宫颈癌患者化疗方案制定和提示预后积累资料。方法 :集子宫颈切除及活检标本 118例 ,其中有正常宫颈组织 13例 ;子宫颈上皮内瘤变 (cervicalintraepithelialneoplasia ,CIN) 2 5例 ;子宫颈鳞状细胞癌 80例。在鳞癌组中高分化鳞癌 13例、中分化鳞癌 5 3例、低分化鳞癌 14例。以S -P免疫组织化学方法 ,显示HSP2 7和MT的表达水平。结果 :染色显示细胞核或细胞浆被染呈大小不一 ,深浅不等的棕黄色颗粒。HSP2 7在正常宫颈上皮、CIN和宫颈鳞癌中阳性率分别为 7.6 9%、2 4 .0 0 %和4 6 .2 5 % ,三组间差异有统计学意义 (P <0 .0 1)。在正常宫颈中MT阳性率为 7.6 9% (1/ 13) ,CIN中阳性率为 2 8.0 0 % (7/ 2 5 ) ,在宫颈鳞癌中阳性率为 5 6 .2 5 % (45 / 80 ) ,三者间有显著性差异 (P <0 .0 1)。HSP2 7和MT的阳性表达率在不同分化程度的宫颈鳞癌间无显著差异。结论 :HSP2 7和MT在正常宫颈、宫颈上皮内瘤变、宫颈鳞癌的阳性表达率逐渐升高 (P <0 .0 1)。HSP2 7和MT的过度表达可分别或联合作为临床上早期诊断宫颈癌或判断CIN预后的参考指标
OBJECTIVE: To investigate the expression of HSP70 and metallothionein in the process of cervical carcinogenesis, to guide the development of chemotherapy regimens for patients with cervical cancer and suggest the accumulation of prognosis data. Methods: A total of 118 cases of cervical resection and biopsy specimens were collected, including 13 cases of normal cervical tissue, 25 cases of cervical intraepithelial neoplasia (CIN) and 80 cases of cervical squamous cell carcinoma. In squamous cell carcinoma group, there were 13 cases of well-differentiated squamous cell carcinoma, 53 cases of moderately differentiated squamous cell carcinoma and 14 cases of poorly differentiated squamous cell carcinoma. S-P immunohistochemistry showed HSP2 7 and MT expression levels. RESULTS: Staining showed that nuclei or cytoplasm were stained with brownish-yellow particles of varying sizes and shades. The positive rates of HSP2 7 in normal cervical epithelium, CIN and cervical squamous cell carcinoma were 7.69%, 24.0% and 46.5% respectively, with significant difference between the three groups (P <0.01) ). The positive rate of MT in normal cervix was 7.69% (1/13), the positive rate in CIN was 8.0% (7/25), and the positive rate in cervical squamous cell carcinoma was 56.2% (45/45) 80), there was a significant difference among the three (P <0.01). The positive rates of HSP27 and MT in cervical squamous cell carcinoma with different degrees of differentiation were not significantly different. Conclusion: The positive rates of HSP27 and MT in cervical cancer, cervical intraepithelial neoplasia and cervical squamous cell carcinoma are gradually increased (P <0.01). Overexpression of HSP27 and MT can be used as a reference index for early diagnosis of cervical cancer or prognosis of CIN