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目的:观察加减补肺汤对慢性阻塞性肺病(COPD)肺气虚证大鼠血浆中内皮素(ET)及一氧化氮(NO)含量,探讨其作用机制。方法:Wistar大鼠随机分出正常组20只,其余80只采用烟熏复合木瓜蛋白酶雾化吸入法造模。将造模成功的大鼠分为模型组、加减补肺汤高、低剂量组(20,10 g.kg-1)、桂龙咳喘宁组(1.125 g.kg-1);正常组和模型组(0.9%氯化钠溶液10 mL.kg-1);5组均于停止烟熏当天开始灌胃,连续15 d,用放免法测定血浆ET,NO含量,并进行组间比较。结果:与模型组比较,各治疗用药组大鼠血浆ET含量降低、NO含量升高(P<0.05),加减补肺汤高剂量组血浆ET含量明显降低(178.98±4.86)ng.L-1,P<0.05;NO显著升高(37.03±0.83)μmol.L-1,P<0.05。结论:加减补肺汤能显著提高血浆NO含量,降低ET含量,调节血管内皮功能,在一定程度上控制慢性炎症反应,延缓肺动脉高压的形成。
Objective: To observe the effect of Jiajianbufei decoction on plasma endothelin (ET) and nitric oxide (NO) contents in rats with chronic obstructive pulmonary disease (COPD) and its mechanism. Methods: Twenty Wistar rats were randomly divided into normal group (n = 20) and the remaining 80 animals were treated with smoked compound papain inhalation method. The model rats were divided into model group, high and low BuBu Bu Tang (20,10 g.kg-1), Guilong Kechuanning group (1.125 g.kg-1); normal group And model group (0.9% sodium chloride solution 10 mL.kg-1). All the rats in the 5 groups were given gavage on the day of stopping smoking for 15 days. The content of ET and NO in plasma were determined by radioimmunoassay and compared among groups. Results: Compared with the model group, the content of ET in plasma decreased and the content of NO increased in each treatment group (P <0.05). The ET level of high dose and high dose group was significantly lower (178.98 ± 4.86) ng.L- 1, P <0.05; NO significantly increased (37.03 ± 0.83) μmol.L-1, P <0.05. Conclusion: Jiajianbufei decoction can significantly increase plasma NO content, decrease ET content, regulate endothelial function, and control chronic inflammatory reaction to a certain extent, retarding the formation of pulmonary hypertension.