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目的:探讨桑菊饮对内毒素(lipopolysaccharide,LPS)诱导小鼠的急性肺损伤(acute lung injury,ALI)保护作用。方法:于小鼠腹腔注射LPS(5 mg/kg)复制ALI动物模型。将小鼠随机分为空白组对照组、LPS组、地塞米松组(2 mg/kg)、桑菊饮水提物组(6.5g/kg、13g/kg)。观察各组肺组织病理学改变,测量肺湿/干重比,支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、炎症因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量及肺组织中p38MAPK与NFκ-BP65蛋白表达。结果:桑菊饮组(6.5 g/kg、13g/kg)可有效减轻LPS所致肺组织病理学变化,能降低肺泡灌洗液中炎症细胞的数目,能显著降低肺湿/干重比重,能提高SOD水平,并能降低MDA水平,减低p38MAPK与NFκBP65蛋白。结论:桑菊饮6.5 g/kg、13g/kg可减轻LPS所致急性肺组织损伤,对LPS诱导的急性肺损伤有保护作用。
Objective: To investigate the protective effect of Sang Ju Yin on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods: Mouse model of ALI was established by intraperitoneal injection of LPS (5 mg / kg). The mice were randomly divided into control group, LPS group, dexamethasone group (2 mg / kg), Sang Ju water extract group (6.5g / kg, 13g / kg). The pathological changes of lung tissue were observed in each group. The wet / dry ratio of lung, the percentage of neutrophil in bronchoalveolar lavage fluid, the content of superoxide dismutase (SOD), malondialdehyde (MDA), interleukin - 6 (IL-6), tumor necrosis factor-α (TNF-α) and the expression of p38MAPK and NFκ-BP65 in lung tissue. Results: Sangjuyin group (6.5g / kg, 13g / kg) can effectively reduce the pathological changes of lung tissue induced by LPS, reduce the number of inflammatory cells in BALF, and can significantly reduce the proportion of lung wet / dry weight, Can increase the level of SOD, and can reduce the level of MDA, reduce p38MAPK and NFκBP65 protein. Conclusion: Sangju Decoction 6.5g / kg, 13g / kg can reduce acute lung injury induced by LPS, which has a protective effect on LPS-induced acute lung injury.