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为了探讨钠氢交换蛋白-1(NHE-1)抑制剂二甲基氨氯吡咪(DMA)对HL-60/ADM细胞pHi变化及细胞增殖、凋亡的影响,以敏感HL-60细胞为对照,用不同浓度的DMA干预后,荧光指示剂(BCECF/AM)检测法检测细胞内pHi,微量噻唑蓝法(MTT)检测细胞生长的抑制率,流式细胞术检测细胞周期变化,TUNEL法检测原位细胞凋亡,对比HL-60/ADM细胞与HL-60细胞之间的差异。结果显示:HL-60/ADM细胞内pHi较HL-60细胞显著升高;DMA作用下,HL-60/ADM细胞的pHi降低幅度、生长抑制率、细胞凋亡率均显著高于HL-60细胞;当DMA浓度100-150μmol/L时,HL-60/ADM细胞的G0/G1期细胞比率增加幅度、S期及G2/M期细胞比率降低幅度均高于HL-60细胞。结论:NHE-1特异性抑制剂DMA引起HL-60/ADM的pHi降低,可抑制细胞生长并诱导细胞凋亡;且DMA对HL-60/ADM细胞的生长抑制作用显著高于HL-60细胞。
In order to investigate the effect of dimethylamilothymidine (DMA), a sodium-hydrogen exchange protein-1 (NHE-1) inhibitor, on the pHi and cell proliferation and apoptosis of HL-60 / ADM cells, Control, with different concentrations of DMA intervention, fluorescence indicator (BCECF / AM) detection of intracellular pHi, trace thiazolyl blue (MTT) detection of cell growth inhibition, flow cytometry to detect cell cycle changes, TUNEL method The apoptosis of HL-60 / ADM cells and HL-60 cells was compared between the two groups. The results showed that the pHi of HL-60 / ADM cells was significantly higher than that of HL-60 cells. Under the action of DMA, the decrease of pHi, the growth inhibition rate and the apoptosis rate of HL-60 / ADM cells were significantly higher than those of HL-60 Cells. When the concentration of DMA was 100-150μmol / L, the percentage of cells in G0 / G1 phase of HL-60 / ADM cells increased, while the percentage of cells in S phase and G2 / M phase decreased more than that of HL-60 cells. CONCLUSION: DMA, a specific inhibitor of NHE-1, can decrease the pHi of HL-60 / ADM and inhibit the growth of HL-60 / ADM cells and induce the apoptosis of HL-60 / ADM cells. .