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本文用氧电极技术测定小鼠肝脏和肌肉组织的耗氧量.将组织放置在台氏液,10%甘油台氏液或二甲亚砜台氏液内,降温到0℃,-20℃,-40℃及-196℃后,迅速在40℃水浴内复温,然后分别测定冷冻前后的耗氧量.实验结果表明,不加冷冻保护剂的肝脏或肌肉组织经过冷冻处理后,呼吸强度随温度的降低而减少.在-20℃时急骤降低,肝脏只有对照组的51%,肌肉为22%.添加冷冻保护剂的冷冻各组,都比不加保护剂的耗氧量要高. 二甲亚砜保护小鼠肝脏和肌肉组织的代谢活力要比甘油在同样条件下为优. 尤其在细胞低温致死的危险温度区域时更为明显.
Oxygen consumption in liver and muscle tissue of mice was measured by oxygen electrode technology.The tissues were placed in Tyrode’s solution, 10% glycerol Tyrosine solution or dimethylsulfoxide in Tyrode’s solution, cooled to 0 ° C, -20 ° C, -40 ℃ and -196 ℃, then quickly rewarmed in water bath at 40 ℃, and then measured the oxygen consumption before and after freezing.The experimental results show that without freezing protective agent of the liver or muscle tissue after freezing, the respiratory intensity with The temperature decreased and decreased sharply decreased at -20 ℃, the liver was only 51% of the control group, the muscle was 22% .In addition of the cryoprotectants in the frozen group, than those without protective agent, the oxygen consumption is higher. Methanesulfone protects mouse liver and muscle tissue metabolites than glycerol under the same conditions as excellent, especially in the temperature of the cell at low temperature lethal temperature is more obvious.