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目的优选檀香叶中牡荆苷与异牡荆苷的提取和纯化工艺。方法以牡荆苷与异牡荆苷的提取率为指标,通过正交试验考察料液比、提取时间、乙醇体积分数、提取次数等因素的影响,优选提取工艺。通过单因素试验考察影响大孔吸附树脂的吸附率、解析率的多种因素,优选檀香叶中牡荆苷与异牡荆苷的纯化工艺。结果优选所得提取工艺为50℃恒温超声提取,料液比1∶8,提取时间为40 min,提取溶剂为60%(V∶V)乙醇,提取次数为3次。纯化工艺为AB-8大孔吸附树脂,上样液浓度为0.25 g/ml(生药量/溶剂);洗脱顺序为:10%乙醇除杂,洗脱体积约10~12 BV(至流出液无色),再换用30%乙醇,洗脱6 BV,洗脱速度为2 BV/h。结论提取与纯化工艺操作简便、稳定可行。
Objective To optimize the extraction and purification of vitexin and isoflavone in sandalwood leaves. Methods Taking the extraction rate of vitexin and isovitexin as indexes, the effects of factors such as material-liquid ratio, extraction time, ethanol volume fraction and extraction times were investigated by orthogonal test, and the extraction process was optimized. Through the single factor test to examine macroporous adsorption resin adsorption rate, resolution rate of a variety of factors, preferably sandalwood leaf vitexin and isovitin purification process. Results The optimal extraction process was ultrasonic extraction at 50 ℃, the ratio of material to liquid was 1:8, the extraction time was 40 min, the extraction solvent was 60% (V: V) ethanol and the extraction times were three times. The purification process was AB-8 macroporous resin. The sample concentration was 0.25 g / ml (crude drug / solvent); the elution order was: 10% ethanol impurity removal, and the elution volume was about 10-12 BV Colorless), and then replaced with 30% ethanol, elution 6 BV, the elution rate of 2 BV / h. Conclusion Extraction and purification process is simple, stable and feasible.