论文部分内容阅读
目的探讨微生态制剂防治非酒精性脂肪性肝炎的作用机理。方法雄性SD大鼠50只随机分组为5组,每组10只,正常组普通饲料喂养;模型Ⅰ组、Ⅱ组喂高脂饲料;微生态制剂治疗组和饮食组分别在喂饲高脂饲料12周末予美常安灌胃和改为普通饲料喂养。12周末处死正常组、模型Ⅰ组大鼠;剩余2组大鼠继续喂养至16周末处死,测定血清转氨酶(ALT、AST),肿瘤坏死因子α(TNFα)水平,肝匀浆丙二醛(MDA)含量,超氧化物歧化酶(SOD)活性,总抗氧化能力(T-AOC),一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)及谷胱甘肽(GSH)水平,观察肝组织学改变。结果模型组大鼠12周出现单纯性脂肪肝,16周发展为脂肪性肝炎。与正常组比,模型组大鼠肝组织脂质过氧化产物MDA含量明显增多(6.45±1.07,8.38±1.32μmol/gvs5.08±0.91μmol/g,P<0.01),而抗氧化物SOD(171±14,148±26kNU/gtvs198±25kKU/gt,P<0.05和P<0.01),GSH(40.8±5.1,35.0±9.0mg/gvs48.5±7.6mg/g,P<0.5和P<0.01)含量明显降低,且肝脏的脂肪变性严重程度随着高脂饮食喂养的时间延长而加剧,微生态制剂治疗组大鼠各项指标较模型组有明显改善(P<0.05或P<0.01),脂肪变性程度明显减轻,而饮食治疗组大鼠各项指标与模型组比无显著差异。结论微生态制剂美常安可能通过减轻体重,改善机体脂质代谢紊乱,抗脂质过氧化反应,抗炎等综合作用来防治非酒精性脂肪性肝炎。
Objective To explore the mechanism of probiotics in the prevention and treatment of non-alcoholic steatohepatitis. Methods Fifty male Sprague-Dawley rats were randomly divided into five groups, 10 in each group. The normal group was fed with normal diet. The model group Ⅰ and group Ⅱ were fed with high fat diet. The probiotics group and the diet group were fed with high fat diet 12 weekend to the United States gavage and changed to normal feed. The rats in the normal group and the model group were sacrificed on the 12th week. The rats in the remaining two groups were sacrificed until the end of the 16th week. The serum levels of ALT, TNF, TNFα and malondialdehyde (MDA) ), Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), nitric oxide (NO), inducible nitric oxide synthase (iNOS) and glutathione (GSH) Liver histology was observed. Results In the model group, simple fatty liver appeared at 12 weeks and steatohepatitis at 16 weeks. Compared with normal group, the content of MDA in liver tissue of model group increased significantly (6.45 ± 1.07,8.38 ± 1.32μmol / g vs5.08 ± 0.91μmol / g, P <0.01), while the content of antioxidant SOD 171 ± 14,148 ± 26 kNU / gtvs198 ± 25 kKU / gt, P <0.05 and P <0.01), GSH (40.8 ± 5.1,35.0 ± 9.0 mg / g vs 48.5 ± 7.6 mg / g, P < (P <0.05 or P <0.01), and the severity of steatosis in the liver aggravated with the prolongation of high-fat diet. Compared with the model group, the indicators in the probiotics group were significantly improved (P <0.05 or P <0.01) The degree of significantly reduced, while the diet treatment group rats and the model group indicators no significant difference. Conclusions Mei-An-An could protect against non-alcoholic steatohepatitis by reducing body weight, improving lipid metabolism, anti-lipid peroxidation and anti-inflammatory.