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以基础饲料为对照组,在基础饲料中分别添加坚强芽孢杆菌活菌(Bacillus firmus)、坚强芽孢杆菌活菌(1.0×108 CFU/g)+美人鱼发光杆菌(Photobacterium damsela)灭活菌(1%)、坚强芽孢杆菌活菌(1.0×108 CFU/g)+溶藻弧菌(Vibrio alginolyticus)灭活菌(1%)配制3种免疫饲料。每组3个重复,对个体质量为(3.2±0.26)g的凡纳滨对虾(Litopenaeus vannamei)进行了为期30 d的养殖实验。每5 d取样,以血清中的酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了肠道益生菌及其灭活菌体作为免疫制剂对凡纳滨对虾非特异性免疫水平的影响;在投喂免疫饲料后的第16天,按0.9 g/10尾剂量,直接投喂感染白斑综合征病毒(WSSV)对虾病料,计算各实验组每天的累计死亡率,分析肠道益生菌及其灭活菌体作为免疫制剂对凡纳滨对虾病毒感染能力的影响。结果显示:添加益生菌的实验组对虾血清中SOD、ACP、AKP和NOS活性明显高于对照组(P<0.05),特别是显著提高了对虾抗WSSV感染的能力。其中坚强芽孢杆菌活菌(1.0×108 CFU/g)和美人鱼发光杆菌灭活菌(1%)实验组的抗病毒感染能力最强,感染WSSV 14 d后累计死亡率为10.71%;而对照组为64.28%。结论认为,饲料中添加肠道益生菌及其灭活菌体能提高凡纳滨对虾非特异性免疫水平和抵抗疾病的能力,有望作为新型对虾免疫增强剂应用于对虾养殖业。
Bacillus firmus, B. firmus (1.0 × 108 CFU / g) + Photobacterium damsela inactivated bacteria (1%) were added to the basal diet respectively. ), Viable Bacillus firmus (1.0 × 108 CFU / g) and Vibrio alginolyticus (1%). Three replicates of each group were used for a 30-day culture experiment of Litopenaeus vannamei with an individual mass of (3.2 ± 0.26) g. Samples were taken every 5 days and immunized with serum ACP, AKP, NOS, SOD and lysozyme (UL) Indicators to explore the intestinal probiotics and inactivated bacteria as immune agents on the non-specific immunity of vannamei shrimp; on the 16th day after immunization feed, according to 0.9 g / 10 tail dose, direct investment Feeding white spot syndrome virus (WSSV) prawn material, calculating the cumulative daily mortality of each experimental group, and analyzing the effect of probiotics and their inactivated bacterial cells as an immunological agent on the infection ability of Litopenaeus vannamei. The results showed that the activity of SOD, ACP, AKP and NOS in the serum of shrimp fed the probiotics group was significantly higher than that of the control group (P <0.05), especially significantly increased the resistance to WSSV infection. The experimental group with viable Bacillus firmus (1.0 × 108 CFU / g) and inactivated metobacter (1%) had the strongest anti-virus infection and the cumulative mortality after WSSV infection for 14 days was 10.71% Is 64.28%. The conclusion is that the addition of probiotics and their inactivated bacteria to feed can increase the non-specific immunity and resistance to disease of shrimp Litopenaeus vannamei, which is expected to be applied to the shrimp aquaculture industry as a new type of shrimp immunity enhancer.