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利用MTT法研究了钨系纳米颗粒对HepG2肝肿瘤细胞抑制作用,利用瞬态吸收谱(TAS)技术的吸收发射谱表征肿瘤细胞和纳米粒子之间的电荷传递,讨论了钨系纳米颗粒在肿瘤疾病早期检测以及抗肿瘤特性。设计HepG2实验体系中,抗肿瘤活性的最佳药物浓度为100~150μg·mL-1,反应时间在6h之内很强,在24h之内已经基本完成。实验证实,瞬态吸收谱技术可以作为肿瘤的检测方法,及表征纳米生物传感器和肿瘤细胞之间的电荷传递,钨系纳米颗粒具有作为抗肿瘤药物的应用前景。
The inhibitory effect of tungsten nanoparticles on HepG2 hepatic tumor cells was studied by MTT method. The charge transfer between tumor cells and nanoparticles was characterized by absorption emission spectroscopy using transient absorption spectroscopy (TAS) Early detection of disease and anti-tumor properties. The optimal drug concentration of anti-tumor activity in HepG2 experimental system was designed to be 100-150 μg · mL-1. The reaction time was very high within 6 h and was basically completed within 24 h. Experiments show that the transient absorption spectroscopy technique can be used as a tumor detection method, and characterize the charge transfer between the nano-biosensor and tumor cells, tungsten-based nanoparticles have the potential as anti-tumor drugs.