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目的 :探讨 APL 细胞经过 ATRA诱导后细胞因子分泌水平的变化。方法 :APL 患者的骨髓和外周血标本被收集于肝素抗凝管中 ,采用传统 Ficoll密度梯度法分离 APL 细胞。按照 FAB细胞形态学和细胞遗传学进行 APL 诊断。采用 EL ISA法测定原代白血病细胞培养悬液中的 IL- 1β、IL- 6、IL- 8、TNFα和 G- CSF含量。NBT还原实验检测 APL 细胞分化状态。结果 :APL 细胞在 ATRA10 - 6 m ol/L体外作用 96 h,或体内诱导作用 96 h,IL - 1β(P<0 .0 5 )和 G- CSF(P<0 .0 5 )分泌水平增加 ,IL - 6和 IL - 8水平降低 (P<0 .0 5 ) ,但 TNFα水平改变不明显。另外 ,体外 APL细胞的增生率与IL - 1β分泌率和 G- CSF呈正相关 ,检测到 IL - 1β或 G- CSF的患者的细胞数明显高于检测不到者。结论 :IL - 1β和 G- CSF在 ATRA作用后的 APL细胞的增生中起重要作用
Objective: To investigate the changes of cytokine secretion of APL cells induced by ATRA. Methods: The bone marrow and peripheral blood samples of patients with APL were collected in heparin anticoagulant tubes. APL cells were isolated by traditional Ficoll density gradient method. APL diagnosis according to FAB cell morphology and cytogenetics. IL-1β, IL-6, IL-8, TNFα and G-CSF in primary leukemia cell culture suspension were measured by ELISA. NBT reduction assay APL cell differentiation status. Results: The secretion of IL - 1β (P <0. 05) and G - CSF (P <0. 05) in APL cells was increased in 96 h after ATRA10-6 mol / L in vitro or in vivo for 96 h , IL - 6 and IL - 8 levels decreased (P <0.05), but TNFα levels did not change significantly. In addition, the proliferation rate of APL cells in vitro was positively correlated with the secretion rate of IL - 1β and G - CSF, and the number of cells with IL - 1β or G - CSF detected was significantly higher than those without. Conclusion: IL - 1β and G - CSF play an important role in the proliferation of APL cells after ATRA treatment