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目的探讨1,25-二羟基维生素D3(VD3)干预前后糖尿病肾病患者外周血单核细胞促炎细胞因子的表达情况。方法纳入2型糖尿病合并糖尿病肾病(DN)患者(DN组)及健康对照者(对照组)各15例,分别检测两组外周血单核细胞培养上清液在VD3干预和非干预条件下白介素(IL)-17A、干扰素(IFN)-γ、肿瘤坏死因子(TNF)及IL-2水平。结果VD3干预前,两组IL-17A水平比较差异无统计学意义(P>0.05),DN组IFN-γ、TNF和IL-2水平均高于对照组(P<0.05);VD3干预后,两组IL-17A、IFN-γ、TNF和IL-2水平均低于干预前,且DN组IL-17A、IFN-γ和IL-2水平仍高于对照组,但TNF水平低于对照组(均P<0.05)。结论相关促炎细胞因子可能在DN发生中起促进作用,VD3对DN患者外周血单核细胞相关促炎因子具有抑制作用。
Objective To investigate the expression of pro-inflammatory cytokines in peripheral blood mononuclear cells of patients with diabetic nephropathy before and after 1,25-dihydroxyvitamin D3 (VD3) intervention. Methods Fifteen patients with type 2 diabetes mellitus (DN) and healthy controls (control group) were enrolled in this study. Peripheral blood mononuclear cell culture supernatants were tested for interleukin (IL) -17A, interferon (IFN) -γ, tumor necrosis factor (TNF) and IL-2. Results The levels of IFN-γ, TNF and IL-2 in DN group were significantly higher than those in control group before VD3 intervention (P> 0.05). After VD3 intervention, The levels of IL-17A, IFN-γ, TNF and IL-2 in two groups were lower than those before intervention, and the levels of IL-17A, IFN-γ and IL-2 in DN group were still higher than those in control group (All P <0.05). Conclusions The related proinflammatory cytokines may play a role in the pathogenesis of DN. VD3 can inhibit the monocyte-associated pro-inflammatory cytokines in peripheral blood of DN patients.