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目的:探讨结直肠差异表达长链非编码RNA CRNDE(colorectal neoplasia differentially expressed)在非小细胞肺癌(NSCLC)组织标本中的表达及其临床意义。方法:收集2011年1月至2015年12月成都军区总医院行根治性或姑息性切除术的NSCLC患者137例,以相应癌旁组织和29例肺外伤非肺癌患者的正常肺组织作为对照,通过实时荧光定量PCR法检测lncRNA CRNDE在癌组织及癌旁组织标本中的表达,分析其表达与患者临床病理特征及预后的关系。结果:lncRNA CRNDE在肺癌癌组织中的平均表达水平为5.283±0.245,与非肿瘤组织(1.098±0.082)比较,差异有统计学意义(t=14.59,P<0.01)。lncRNA CRNDE在肺癌组织标本中的表达较癌旁组织明显增高。lncRNA CRNDE的表达与患者的年龄、性别、吸烟史、肿瘤大小、淋巴结转移及肿瘤分化无关(均P>0.05);与疾病分期、远处转移、病理类型及生存状态明显相关(均P<0.05)。lncRNA CRNDE高表达水平与NSCLC中的病理类型相关(P<0.05),在腺癌组的表达水平明显高于鳞癌及其他类型组的表达水平;此外,高表达lncRNA CRNDE的患者的PFS为(20.00±1.72)个月,较低表达者(34.07±1.97)缩短,差异有统计学意义(χ~2=15.940,P<0.01);低表达lncRNA CRNDE的患者的OS为(47.50±2.31)个月,较高表达者(32.15±2.19)明显延长,差异有统计学意义(χ2=12.40,P<0.01)。Cox多因素回归模型分析显示,lncRNA CRNDE的表达、远处转移及临床分期是NSCLC独立的预后因素(P<0.05)。结论:lncRNA CRNDE参与调节NSCLC的发生发展,可作为潜在的NSCLC诊断和预后评估的生物标志物。
Objective: To investigate the expression of colorectal neoplasia differentially expressed (CRNDE) in non-small cell lung cancer (NSCLC) and its clinical significance. Methods: A total of 137 NSCLC patients who underwent radical or palliative resection from January 2011 to December 2015 in Chengdu Military General Hospital were enrolled. The normal lung tissues of corresponding para-cancer tissues and 29 lung-injury non-lung cancer patients were collected as controls. The expression of lncRNA CRNDE was detected by real-time fluorescence quantitative PCR in cancer tissues and adjacent normal tissues. The relationship between the expression of CRNs and clinicopathological characteristics and prognosis was analyzed. Results: The average expression level of lncRNA CRNDE in lung cancer tissues was 5.283 ± 0.245, which was significantly different from that in non-tumor tissues (1.098 ± 0.082) (t = 14.59, P <0.01). The expression of lncRNA CRNDE in lung cancer tissues was significantly higher than that in paracancerous tissues. The expression of lncRNA CRNDE was not associated with the age, sex, smoking history, tumor size, lymph node metastasis and tumor differentiation (all P> 0.05), but significantly correlated with the stage, distant metastasis, pathological type and survival status ). The high expression of lncRNA CRNDE correlated with pathological types in NSCLC (P <0.05), and was significantly higher in adenocarcinoma than that in squamous cell carcinoma and other types. In addition, the PFS in patients with highly expressed lncRNA CRNDE was ( 20.00 ± 1.72) months, and the lower expression was (34.07 ± 1.97), the difference was statistically significant (χ ~ 2 = 15.940, P <0.01); the OS of patients with low expression of lncRNA CRNDE was (47.50 ± 2.31) Month, higher expression (32.15 ± 2.19) was significantly longer, the difference was statistically significant (χ2 = 12.40, P <0.01). Cox regression analysis showed that the expression of lncRNA CRNDE, distant metastasis and clinical stage were independent prognostic factors of NSCLC (P <0.05). CONCLUSION: lncRNA CRNDE is involved in the regulation of the development of NSCLC and may serve as a potential biomarker for the diagnosis and prognosis of NSCLC.