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热休克蛋白(heat shock proteins,Hsps)是生物体或细胞受到热胁迫后新合成的一类遗传上高度保守的蛋白,在昆虫应对外界环境因子胁迫时起着重要作用。为了系统研究棉花粉蚧Phenacoccus solenopsis Hsp基因家族,对棉花粉蚧转录组基因注释信息进行分析、获得目标序列,并应用NCBI上Blast X等软件进行比对、共鉴定出24条热激蛋白(Hsp)基因,包括3个Hsp90、8个Hsp70、2个Hsp60和11个s Hsp(small heat shock protein,s Hsp)基因。对棉花粉蚧与模式昆虫家蚕Bombyx mori、黑腹果蝇Drosophila melanogaster、赤拟谷盗Tribolium castaneum系统进化关系分析显示,昆虫的小分子量热休克蛋白s Hsp具有很强的种属特异性,Hsp70家族的保守性比s Hsp强。棉花粉蚧热激蛋白基因的鉴定为深入研究该虫Hsp与生长发育、抗逆境的相互关系奠定了基础。
Heat shock proteins (Hsps) are a newly synthesized class of genetically highly conserved proteins that are exposed to heat stress in organisms or cells and play an important role in coping with environmental stressors. In order to systematically study Phenacoccus solenopsis Hsp gene family in cotton ticks, we analyzed the gene annotation information of cotton ticks, obtained the target sequences and compared them with software Blast X on NCBI to identify 24 heat shock proteins (Hsp ) Genes including 3 Hsp90, 8 Hsp70, 2 Hsp60 and 11 small heat shock protein (s Hsp) genes. The phylogenetic analysis of cotton mealybugs with the model insects Bombyx mori, Drosophila melanogaster and Tribolium castaneum showed that the insect small-molecular-weight heat shock protein s Hsp has strong species-specific characteristics. The Hsp70 family Conservative than s Hsp strong. Identification of cotton mealyblases heat shock protein gene laid a foundation for further study on the relationship between Hsp and growth, development and stress resistance.