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目的:建立小檗根药材的质量控制方法。方法:采用显微鉴别法鉴别小檗根药材粉末的显微特征;采用薄层及高效液相色谱法鉴别及测定盐酸巴马汀及盐酸小檗碱。结果:制定了小檗根药材显微鉴别方法;制定了薄层鉴别方法及高效液相色谱法测定两种生物碱含量。结论:以上3种方法简便、准确,适用于小檗根药材的质量控制。
Objective: To establish the quality control method of Berberis root medicine. Methods: Microscopic identification was used to identify the microscopic characteristics of Berberine root powder. TLC and HPLC were used to identify and determine the effects of palmatine hydrochloride and berberine hydrochloride. Results: The microscopic identification method of Berberis root medicine was established. TLC method and HPLC were used to determine the contents of two alkaloids. Conclusion: The above three methods are simple, accurate and suitable for the quality control of Berberis root medicine.