论文部分内容阅读
Ｒ２８４．１；Ｒ３３１．３８；Ｒ９７２．２；Ｒ９７２．４摘要目的观察Ｒｂ１对豚鼠心肌细胞膜Ｌ－型电压依赖性钙通道的影响。方法采用标准全细胞膜片钳技术（ｗｈｏｌｅ－ｃｅｌｐａｔｃｈｃｌａｍｐｒｅｃｏｒｄｉｎｇｔｅｃｈｎｉｑｕｅ）。结果在保持电位－４０ｍＶ，细胞去激化至＋４０ｍＶ，刺激频率０．５Ｈｚ，时程１５０ｍｓ条件下，Ｒｂ１１０μｍｏｌＬ－１和３０μｍｏｌＬ－１分别使ＢａｙＫ８６４４和ｎｉｆｅｄｉｐｉｎｅ敏感的钙内向电流减少１６．２％±３．７％（Ｐ＜０．０５，ｎ＝５）和３８．３％±１０．４％（Ｐ＜０．０１，ｎ＝５），且在３～１０００μｍｏｌＬ－１范围内，其抑制作用呈浓度依赖关系。结论实验证明Ｒｂ１为一钙通道阻滞剂。
R284.1; R331.38; R972.2; R972.4 Abstract Objective To observe the effect of Rb1 on L-type voltage-dependent calcium channels in guinea pig cardiomyocytes. Methods A standard whole-cell patch clamp technique (whole-cel patch clamp imaging) was used. RESULTS: At a holding potential of -40 mV, the cells were desensitized to +40 mV, the stimulation frequency was 0.5 Hz, and the time course 150 ms, Rb 110 μmol L-1 and 30 μmol L-1, respectively, reduced BayK8644 and nifedipine-sensitive calcium inward current by 16.2%±3. 7% (P<0.05, n=5) and 38.3%±10.4% (P<0.01, n=5), and in the range of 3 to 1000 μmol L-1, the inhibitory effect was concentration Dependencies. Conclusions Experiments have shown that Rb1 is a calcium channel blocker.