论文部分内容阅读
以新疆野生樱桃李(Prunus sogdiana Vassilcz.)扦插苗为试材,接种南方根结线虫(Meloidogyne incognita)鉴定其抗性,并对抗病机理进行初步分析。结果显示:新疆野生樱桃李抗病植株发生过敏性(HR)反应,在接种后1d根系就出现典型的HR反应,并且在线虫周围细胞中发生HR反应的比例始终高于感病植株。在抗性植株发生HR反应的同时其体内大量产生过氧化氢,尤其在接种后1~2d过氧化氢和超氧阴离子的积累快速增加,这与HR反应特征基本一致。在接种南方根结线虫后,抗病植株中的VAD1和PO2表达均高于感病植株,说明新疆野生樱桃李抗南方根结线虫的机制在于侵染后首先引起过氧化氢和超氧阴离子的上升,进而产生HR反应限制了巨细胞的发育,使线虫得不到足够的营养而发育受阻,导致不能形成根结。同时VAD1和PO2基因表达上调可能启动抗病反应,从而使植株产生抗性。
The seedlings of Prunus sogdiana Vassilcz. Were used as test materials and inoculated with Meloidogyne incognita to test their resistance. The resistance mechanism of Prunus sogdiana Vassilcz. The results showed that there was an allergic reaction (HR) reaction in the resistant plants of wild cherry plum in Xinjiang. Typical HR responses occurred on the 1st day after inoculation, and the proportion of HR responses in the cells around the nematode was always higher than that of the susceptible plants. Hydrogen peroxide was produced in large quantities in the resistant plants when HR reaction occurred. Especially, the accumulation of hydrogen peroxide and superoxide anion rapidly increased 1 to 2 days after inoculation, which was consistent with the HR reaction characteristics. After inoculation with Meloidogyne incognita, the expression of VAD1 and PO2 in the resistant plants was higher than that in the susceptible plants, indicating that the mechanism of the resistance of wild cherry plum to Xinjiang Meloidogyne incognita is that it firstly causes hydrogen peroxide and superoxide anion Rising, resulting in HR response limits the development of giant cells, so that nematodes are not enough nutrition and development blocked, resulting in the formation of root knot. At the same time, the upregulation of VAD1 and PO2 gene may start the disease-resistant reaction, which makes the plant produce resistance.