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目的 :探讨依赖还原型辅酶I/II醌氧化还原酶基因cDNA60 9位C→T点突变所致的基因多态性是否与肺癌遗传多态性有关。方法 :采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)的分析方法分析了 95例肺癌病人与 136例健康成人对照组NQO1的基因多态性。结果 :T等位基因频率在肺癌组和对照组分别为5 4%、43% ,两组有显著差异 (P =0 0 2 4)。基因型分布在肺癌组和对照组之间差异显著 (p =0 0 0 8)。T/C和T/T基因型携带者患肺癌的危险性分别是野生型纯合子 (C/C)的 2 949倍 (95 %可信限 :1 42 6～ 6 0 96 )与 3 10 6倍(95 %可信限 :1 2 84～ 9 5 14)。结论 :本研究结果提示NQO1基因cDNA60 9突变T等位基因可能是肺癌发生的危险性因素 ,与肺癌的遗传易感性有关。
Objective : To investigate whether genetic polymorphisms resulting from 9 C→T point mutations in the cDNA of reduced coenzyme I/II quinone oxidoreductase gene are associated with genetic polymorphisms in lung cancer. Methods : The gene polymorphisms of NQO1 in 95 lung cancer patients and 136 healthy adult controls were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis. Results: The frequency of T allele was 54% in the lung cancer group and 43% in the control group. There was a significant difference between the two groups (P = 0.024). The genotype distribution was significantly different between the lung cancer group and the control group (p =0 08). The risk of lung cancer in T/C and T/T genotype carriers was 2,949-fold (95% confidence limit: 146-6600) and 3 10 6 in wild-type homozygotes (C/C), respectively. Times (95% confidence limit: 1 2 84 to 9 5 14). Conclusion : This study suggests that the NQO1 gene cDNA-609 mutation T allele may be a risk factor for lung cancer and is associated with the genetic susceptibility of lung cancer.