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A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography(HPAEC) has been developed with integrated pulsed amperometric detection(IPAD).Dionex AS 18(250 mm×2 mm) and AG 18 (50 mm x 2 mm) columns and 11 mmol/L NaOH solution were used for separation.Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio(S/N).Utilizing an optimized waveform,the repeatability(intra-day) precision and intermediate(inter-day) precision are obtained with relative standard deviation(RSD) of 1.88,2.27,respectively.The limit of quantification(LOQ) and limit of detection(LOD) were found to be 9.70,3.0 ng/mL,respectively,with correlation coefficients of 0.9992 over concentration range 0.01-10μg/mL.The present method was successfully applied to the determination of zidovudine in human plasma.The recoveries of plasma sample spiked by 0.7μg/mL,2.7μg/mL obtained were 95.3-101.5%, with relative standard deviation(RSD) of 2.54%,2.21%,respectively.
A rapid and practical method for direct detection of zidovudine in high performance anion exchange chromatography (HPAEC) has been developed with integrated pulsed amperometric detection (IPAD) .ionex AS 18 (250 mm × 2 mm) and AG 18 (50 mm × 2 mm ) columns and 11 mmol / L NaOH solution were used for separation. Multi-step potential waveform parameters were optimized to maximize the signal-to-noise ratio (S / N). Utilizing an optimized waveform, the repeatability (intra-day) precision and intermediate (inter-day) precision were obtained with relative standard deviation (RSD) of 1.88,2.27, respectively. The limit of quantification (LOQ) and limit of detection (LOD) were found to be 9.70, 3.0 ng / mL, , with correlation coefficients of 0.9992 over concentration range 0.01-10 μg / mL. The present method was successfully applied to the determination of zidovudine in human plasma. The recoveries of plasma sample spiked by 0.7 μg / mL, 2.7 μg / mL obtained were 95.3- 101.5% with relative standard deviation (RSD) of 2.54%, 2.21%, respectively.