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1985年在河北省红小豆主产区红小豆罹病植株上采集了38份标样,从中分离出8502分离物。二年重复鉴定表明,该分离物可以侵染茄科、豆科、藜科等7科31种植物。体外稳定性測定:钝化温度60°—65℃,稀释限点10~(-3)—10~(-4),体外保毒期(室温)1—2天。病毒可通过摩擦接种传毒。并由桃蚜做非持久性传播。该分离物和黄瓜花叶病毒抗血清呈阳性反应。电镜下病毒形态为球状粒体,大小30nm。根据上述性状判断,8502分离物属于黄瓜花叶病毒(CMV)。用琼脂双扩散法检测该病毒在田间自然感染率约为12.7％—36％。可以认为黄瓜花叶病毒是红小豆病毒病的主要病毒种类之一。
In 1985, 38 samples were collected from the diseased plants of Adzuki Bean in the main producing area of Adzuki Bean in Hebei Province, and 8502 isolates were isolated from them. Two years of repeated identification showed that the isolate can infect Solanaceae, Leguminosae, Chenopodiaceae and other 31 families of 31 species of plants. In vitro stability determination: passivation temperature of 60 ° -65 ℃, dilution limit of 10 -3 (-3) -10 ~ (-4), in vitro protection period (room temperature) 1-2 days. Viruses can be vaccinated by friction. Peach aphid and do non-persistent transmission. The isolate and cucumber mosaic virus antiserum were positive. Under electron microscopy, the morphology of the virus is globular granules, the size of 30nm. According to the above characters to judge, 8502 isolates belong to cucumber mosaic virus (CMV). The natural infection rate of this virus in the field was about 12.7% -36% by agar double diffusion assay. Cucumber mosaic virus can be considered as one of the major virus types of red bean virus disease.