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目的探讨通过小干扰RNA(siRNA)抑制MICA基因表达对胃癌SGC-7901细胞生长的抑制作用。方法将靶向MICA的siRNA(MICA siRNA)通过脂质体转染法瞬时转染胃癌SGC-7901细胞48 h;Western blot检测siRNA转染前后SCG-7901细胞内MICA蛋白表达的变化;四甲基偶氮唑盐(MTT)法检测MICA siRNA转染对细胞增殖的影响,TUNEL检测siRNA转染对细胞凋亡的影响。结果 MICA siRNA转染干扰SCG-7901细胞内MICA表达,并诱导细胞凋亡和抑制细胞增殖。细胞转染后,MICA蛋白表达降低70%~85%,明显低于对照组,差异有统计学意义(P<0.05);转染组细胞存活率明显低于对照组,差异有统计学意义(P<0.05);转染组细胞凋亡率明显高于对照组,差异有统计学意义(P<0.05)。结论靶向MICA抑制胃癌SGC-7901细胞增殖并促进细胞凋亡,MICA可用于胃癌基因治疗的靶点。
Objective To investigate the inhibitory effect of MICA gene expression on the growth of gastric cancer cell line SGC-7901 by small interfering RNA (siRNA). Methods MICA siRNA targeting MICA was transiently transfected into SGC-7901 cells by liposome transfection method for 48 h. Western blot was used to detect the MICA protein expression in SCG-7901 cells before and after transfection. The effect of MICA siRNA transfection on cell proliferation was detected by MTT assay. The effect of siRNA transfection on apoptosis was detected by TUNEL. Results MICA siRNA transfection interfered MICA expression in SCG-7901 cells and induced apoptosis and inhibited cell proliferation. After transfection, the MICA protein expression decreased by 70% -85%, which was significantly lower than that of the control group (P <0.05). The cell survival rate in transfection group was significantly lower than that in control group (P <0.05) P <0.05). The apoptosis rate of transfected group was significantly higher than that of the control group, the difference was statistically significant (P <0.05). Conclusion MICA can inhibit the proliferation of SGC-7901 cells and promote the apoptosis of gastric cancer cells. MICA can be used as a target for gene therapy of gastric cancer.