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Sesame (Sesamue indicum L.) is one of the most important oilseed crops with high oil yield.Here,we described a simple and efficient method for constructing a normalized cDNA library from a high oil content cultivar of sesame Zhongzhi 14,during its oil accumulation stages.It combined switching mechanism at 5′-end of RNA transcript (SMART) technique and duplex-specific nuclease (DSN) normalization methods.Double-stranded cDNAs were synthesized from mRNAs,processed by normalization and Sfi I restriction endonuclease,and finally the cDNAs were ligated to pDNR-LIB vector.The ligation mixture was transformed into Escherichia coli DH10B by electroporation.The capacity of the library was 1.0×106 clones in this library.Gel electrophoresis results indicated the fragments ranged from 700 to 2 000 bp,with the average size of 1 800 bp.Random picking clones showed that the recombination rate was 100%.The results showed that the cDNA library constructed successfully was a full-length library with high quality,and could be used to screen the genes related to development of oil synthesis.
