黔产粗毛淫羊藿与黔岭淫羊藿总黄酮对2型糖尿病小鼠IR相关因子的影响

来源 :中国实验方剂学杂志 | 被引量 : 0次 | 上传用户:jiangjia09
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:研究黔产粗毛淫羊藿与黔岭淫羊藿中总黄酮(TFE)对四氧嘧啶(ALX)诱发2型糖尿病模型小鼠胰岛素抵抗(IR)相关因子的影响。方法:雄性小鼠100只,随机取10只为正常组,喂养基础饲料;其余的采用高脂饲料喂养小鼠6周,单次ip 2%ALX(200 mg·kg-1),建立2型糖尿病小鼠模型。将糖尿病模型小鼠随机分为6组,每组10只,分别为模型组,二甲双胍(150 mg·kg-1)组,两种TFE分别按剂量100,50 mg·kg-1分组,ig给药14 d。于末次给药后取血,检测小鼠血清胰岛素(FINS)、C肽水平;检测小鼠空腹血糖(FBG),注射胰岛素(Ins)40 min后血糖(BG);计算胰岛素耐量(ITT),胰岛素抵抗指数(HOMA-IR)和胰腺指数;观察小鼠胰腺形态学改变。结果:与正常组比较,模型组小鼠血糖升高非常显著(P<0.01),空腹血清中C肽,FINS有明显增加(P<0.05),胰腺组织损伤极为严重(P<0.01),胰腺指数降低非常显著(P<0.01)。与模型组相比,两种淫羊藿TFE均能够较明显地改善模型小鼠ITT(P<0.05),降低模型小鼠血清C肽、FINS含量及HOMA-IR(P<0.05),提高模型小鼠的胰腺指数(P<0.05),明显地修复模型小鼠的胰腺组织损伤(P<0.05)。两种TFE同剂量组相比,以粗毛淫羊藿TFE作用较为显著(P<0.05,P<0.01)。结论:两种黔产淫羊藿TFE对ALX诱生的2型糖尿病模型小鼠能增加胰岛素敏感性,较明显地改善模型小鼠胰岛素抵抗相关因子,保护受损的胰腺组织。以粗毛淫羊藿TFE作用较为明显,这可能与粗毛淫羊藿TFE中淫羊藿苷(ICA)含量高有较大关系。 Objective: To study the effects of total flavonoids (TFE) from Qian epichloa epiphylla and Qianling epimedium on insulin resistance (IR) in type 2 diabetic mice induced by alloxan (ALX). Methods: 100 male mice were randomly divided into normal group and fed with basal diet. The remaining mice were fed with high-fat diet for 6 weeks and were given ip 2% ALX (200 mg · kg-1) Diabetic mouse model. The diabetic mice were randomly divided into 6 groups (n = 10): model group and metformin group (150 mg · kg -1). The two TFE groups were divided into groups of 100 and 50 mg · kg -1 Medicine 14 d. After the last administration, blood samples were taken for determination of serum insulin (FINS) and C-peptide levels; fasting blood glucose (FBG) and blood glucose (BG) were measured after 40 min of insulin injection; insulin tolerance (ITT) Insulin resistance index (HOMA-IR) and pancreatic index were observed. Morphological changes of pancreas were observed. Results: Compared with normal group, the level of C-peptide and FINS in fasting serum of model group was significantly increased (P <0.01), the severity of pancreatic tissue injury was extremely severe (P <0.01) The index decreased significantly (P <0.01). Compared with the model group, both epimedium TFE can significantly improve the model mice ITT (P <0.05), reduce the model mice serum C-peptide, FINS content and HOMA-IR (P <0.05), improve the model The pancreatic index (P <0.05) of mice significantly restored the pancreatic tissue injury in model mice (P <0.05). Compared with the two dosage forms of TFE, the TFE effect of Epimedium brevicornum was more significant (P <0.05, P <0.01). Conclusion: Two kinds of Epimedium brevicornis TFE can increase insulin sensitivity in type 2 diabetes mellitus mice induced by ALX, obviously improve insulin resistance related factors in model mice and protect damaged pancreatic tissues. The effect of TFE on shaggy epimedium was obvious, which may be related to the high content of icariin (ICA) in Epimedium brevicornum.
其他文献
目的:本文运用文献研究与现代统计学分析相结合的方法,系统整理庞安时《伤寒总病论》中关于温热病的条文,分析、归纳其对温热病病名、病因、病机及治法的论述,并进行数据挖掘
目的:通过观察补脾胃泻阴火升阳汤治疗围绝经期综合征的临床治疗效果,探讨脾胃论治在治疗该病的特点和思路,从而为临床上利用中医健脾益气法为主对治疗围绝经期综合征提供可
新媒体环境下风险交流在农产品质量安全风险管理中具有十分重要的作用。本文深入剖析了当前我国农产品质量安全风险交流中存在的几个突出问题,并对此提出了优化策略,以有效提
建立了梯级水电站短期周优化调度的梯级蓄能最大模型,在此基础上采用动态搜索算法对其进行求解。通过严密的理论推导和详尽的实例分析探讨了流域梯级电站负荷最优分配规律。
目的:评估广安门医院维持性血液透析(maintenance hemodialysis,MHD)患者的睡眠质量、症状困扰情况及MHD伴睡眠障碍患者的中医证候分布特点,分析影响维持性血液透析患者睡眠
结合轻轨交通发展历程及现行国家行业标准的界定,从运能、车辆、路权、线路等多方面,对轻轨概念及其与地铁和有轨电车的差异进行了辨析。阐述轻轨系统的主要技术标准与特征,
本文描写韶关本城话一种特殊变音现象,从变音的语音特点、变音字的来源和位置以及变音的语法和词汇意义入手,总结其特点如下:1、变音以韵母中嵌喉塞并变调为话音形式,变音字中有古
目的:本研究旨在通过临床观察糖参乐丸治疗2型糖尿病(气阴两虚证)的临床疗效及其安全性,证明糖参乐丸是治疗此类型糖尿病的有效药物,为临床治疗该病提供一定参考依据。方法:
【目的】通过整理明清时期医家文献,总结出明清医家对遗精辨治的规律并比较其异同点,为中医临床治疗提供参考。【方法】以《明清名医医学全书》系列为基础,结合《中华医典》
以浸渍法制备CuO/硅藻土催化剂,采用XRD、SEM、FTIR、氮气吸附脱附等表征手段对CuO/硅藻土进行表征。以此催化剂催化苯酚双氧水羟化反应合成苯二酚,考察了反应温度、CuO/硅藻