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目的探讨清脑益髓调督法电针治疗脑梗死的作用途径,丰富脑梗死病机研究及针刺干预机制。方法选用健康SD大鼠96只,随机分为正常空白组(假手术组)、阴性对照组(模型组)、电针Ⅰ组(清脑益髓调督组)和电针Ⅱ组(普通电针组)。相应实验处理后,采用RT-PCR检测ephrin B2 mRNA的表达率。结果各组大鼠ephrin B2 mRNA表达率的比较:假手术组各时相上呈弱阳性表达,无显著性差异(P>0.05);模型组表达率随着时间的延长而增高,3d、7d组优于1d组(P<0.05),14d组明显优于1d组及假手术组(P<0.01),7d组表达高于3d组,但无显著性差异(P>0.05);普通电针组,3d、7d、14d组明显优于1d组(P<0.05),以7d组最为显著(P<0.01),7d组与14d组无显著性差异(P>0.05);清脑益髓调督组,7d、14d组优于1d组(P<0.05),治疗3d组明显优于其他三个时相,并且优于普通电针组(P<0.01),7d组与14d组比较,无显著性差异(P>0.05)。结论针刺疗法能够促进血管新生,早期采用清脑益髓调督法治疗急性脑梗死疗效更优,其作用机制可能是通过上调ephrin B2 mRNA的表达,从而促进毛细血管新生、缺血区周围组织灌流和神经功能恢复。
Objective To investigate the mechanism of action of clearing the brain and benefiting the moxibustion in regulating electroacupuncture on cerebral infarction, enrich the mechanism of cerebral infarction and the mechanism of acupuncture intervention. Methods Ninety-six healthy SD rats were randomly divided into two groups: normal control group (sham operation group), negative control group (model control group), EA group Ⅰ Needle group). After corresponding experimental treatment, the expression of ephrin B2 mRNA was detected by RT-PCR. Results The expression of ephrin B2 mRNA in each group was weakly positive in each group at each time point (P> 0.05). The expression rate of model group increased with time, (P <0.05). The levels in 14d group were significantly better than those in 1d group and sham operation group (P <0.01), and those in 7d group were higher than those in 3d group (P> 0.05) (P <0.05), especially in the 7d group (P <0.01). There was no significant difference between the 7d group and the 14d group (P> 0.05) Governor group, 7d, 14d group was better than 1d group (P <0.05), the treatment 3d group was significantly better than the other three phases, and better than the normal electroacupuncture group (P <0.01), 7d group and 14d group, no Significant difference (P> 0.05). Conclusions Acupuncture can promote angiogenesis. Early treatment of acute cerebral infarction with qingnao yi tung myelopathy is more effective. Its mechanism may be through the up-regulation of ephrin B2 mRNA expression, thereby promoting angiogenesis, tissue around the ischemic area Perfusion and nerve function recovery.