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目的了解北京怀柔地区腹泻患者腺病毒感染情况及其分子特征。方法采集怀柔区2013年4月至2014年3月120例腹泻患者粪便标本,用聚合酶链反应(PCR)方法检测腺病毒hexon区域,然后对PCR阳性扩增产物进行测序。采用Mega软件对序列进行比对并构建进化树。结果 120例标本中腺病毒阳性24例,检出率为20.0%;其中7月龄到2岁婴幼儿患者检出率最高,为26.7%(16/60);2个感染高峰分别为6-8月和10-11月,其余月份有腺病毒感染的散发。对其中18例腺病毒hexon区域PCR阳性产物测序和系统进化分析显示,13例为腺病毒F组41型(72.2%,13/18),2例为F组40型(11.1%,2/18),2例为C组的Ad2和Ad6(11.1%,2/18),A组31型1例。结论腺病毒是怀柔地区2013-2014年病毒性腹泻的重要病原之一,F组41型为优势流行株,同时有其他型别的散发。
Objective To understand the adenovirus infection and its molecular characteristics in diarrhea patients in Huairou district of Beijing. Methods Stool specimens from 120 diarrhea patients in Huairou district from April 2013 to March 2014 were collected. The adenovirus hexon region was detected by polymerase chain reaction (PCR) and the positive PCR products were sequenced. The sequences were aligned using Mega software and the phylogenetic tree was constructed. Results The positive rate of adenovirus in 120 cases was 20.0%. The highest detection rate was 26.7% (16/60) in infants from 7 months to 2 years old. The highest infection rates were 6 - August and October-November, the remaining months of adenovirus infection. Sequence analysis and phylogenetic analysis of 18 positive PCR products of hexon in adenovirus showed that 13 cases were adenovirus F group 41 (72.2%, 13/18) and 2 cases were F group 40 (11.1%, 2/18 ), 2 cases of C group Ad2 and Ad6 (11.1%, 2/18), A group of 31 cases of type 1 cases. Conclusion Adenovirus is one of the important pathogens of viral diarrhea in Huairou from 2013 to 2014. F group 41 is the predominant epidemic strain with other types of excretion.