在采用国内习用的胆红素含量测定方法时,除溶剂的选择(参见“胆红素含量测定的溶剂选择”,本刊1987年1期)之外,具体操作方法也是影响测定结果的重要方面。现对有关问题作一比较,并草拟出改进的操作方法。一实验材料 1.胆红素:(1)进口品(瑞典,实验试剂,～99％(uv),λmax453nm,logε4.78,Asche<0.1％)。 (2)国产对照品(北京药品生物制品检定所,810608) In addition to the choice of solvent (see Solvent Selection for Determination of Bilirubin, Issue 1, 1987), the practice of using bilirubin assays in the country is an important aspect of the assay result . We are now comparing the issues and drafting a revised method of operation. Experimental Materials 1. Bilirubin: (1) Imported product (Swedish, experimental reagent, ~ 99% (uv), λ max 453 nm, log ε 4.78, Asche <0.1%). (2) domestic reference substance (Beijing Pharmaceutical and Biological Products Inspection Institute, 810608)