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应用来自体外授精(IVF)或配子移植(GIFT)患者的卵巢颗粒黄体细胞(GL细胞),纯化后培养10日,观察在肿瘤坏死因子α(TNF_α)作用下,对细胞增殖及雌、孕激素分泌的影响。加入重组人TNF_α0.1～10ng/ml后,GL细胞增殖加快,以第0～2日最显著,TNF_α10ng组较对照组增殖一倍。在培养第4～10日TNF10ng组孕激素(P_4)和雌激素(E_2)分泌量增加。若按细胞计每细胞P_4分泌量并无增加而E_2在第8～10日分泌量增加,在培养的GL细胞中加入前列腺素E_2(PGE_2)时,显示PGE_2抑制TNF使GL细胞增殖的作用。提示TNF可促进GL细胞增殖及P_4、E_2分泌增加,并对黄体形成起一定作用。
Ovarian granulosa cells (GL cells) from IVF or GIFT patients were cultured and purified for 10 days. The effects of tumor necrosis factor α (TNF_α) on proliferation and estrogen and progesterone Secretion effects. The addition of recombinant human TNF_α0.1 ~ 10ng / ml, GL cell proliferation accelerated to 0 to 2 days the most significant, TNF_α10ng group than the control group doubled. The secretion of progesterone (P 4) and estrogen (E 2) in TNF10ng group increased from the 4th to 10th day of culture. If the secretion of P 4 per cell did not increase but the secretion of E 2 increased on the 8th to 10th day, the effect of PGE 2 on the proliferation of GL cells was inhibited by the addition of prostaglandin E 2 (PGE 2) to cultured GL cells. These results suggest that TNF can promote the proliferation of GL cells and increase the secretion of P 4 and E 2, and play a role in the formation of corpus luteum.