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随着金针菇杂交育种的深入进行,迫切需要稳定、可靠且便于检测的遗传标记,以用于品系早期鉴定、分类以及种质资源评估等方面.食用菌同工酶标记的研究应用已有许多成功报道(Royse等1982;王贤樵等1989;May等1992).同工酶表达受基因控制,通常在个体发育中表现时空性,在群体中表现出多态性,电泳表型普遍存在生长发育阶段性和特异性(陆士伟等1987;王贤樵等1989;程东升等1995).所以寻找具有稳定性和多态性的同工酶类,即在营养生长阶段或生
With the further development of Flammulina velutipes cross breeding, there is an urgent need for stable, reliable and easy-to-detect genetic markers for the early identification, classification and germplasm evaluation of the strains, etc. There have been many successes in the research and application of mushroom isozymes (Royse et al. 1982; Wang Xianqiao et al. 1989; May et al 1992). Isoenzyme expression is controlled by genes and usually exhibits spatial and temporal properties in individual development and polymorphism in populations. The electrophoretic phenotype generally has stages of growth and development And specificity (Lu Shiwei 1987; Wang Xianqiao 1989; Cheng Dongsheng et al 1995). So looking for stability and polymorphism isozymes, that is, in vegetative growth stage or Health