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为研究内源激素与葡萄休眠的关系,我们首先对脱落酸的分离和测定进行了探索。通过提取、分离、生物鉴定等步骤,得到了具有抑制活性的物质。将该物质以标准脱落酸为对照,并用紫外分光光度计对其做了定性与定量分析。从试验结果可初步确认,于葡萄叶中所提取的具有抑制活性的物质即为脱落酸。现将我们的做法总结如下。
In order to study the relationship between endogenous hormones and grape dormancy, we first explored the separation and determination of abscisic acid. Through the extraction, separation, bioassay and other steps, have inhibitory activity of the material. The substance with standard abscisic acid as a control, and its use of UV spectrophotometer to do a qualitative and quantitative analysis. It can be initially confirmed from the test results that the substance with inhibitory activity extracted from grape leaves is abscisic acid. Now our approach is summarized as follows.