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目的 了解贵州地区TTV感染状况 ,分析TTV贵州株的基因特点。方法 以公布的TTV第 1读码区序列设计两对寡核苷酸引物 ,用套式聚合酶链反应法 (nested PCR)检测贵州地区不同人群血清中TTV核酸 (TTVDNA) ,并对 3份TTVDNA阳性血清的PCR产物 ,用直接测序法测定核苷酸序列。结果 6 2例正常人 ,37例志愿献血员 ,5 0例血液透析患者 ,10 7例静脉药瘾者及 139例肝病患者血清中TTVDNA阳性率分别为 6 .4 5 % (4 6 2 ) ,8.1% (3 37) ,2 6 .0 % (13 5 0 ) ,2 5 .2 3% (2 7 10 7)和16 .5 5 % (2 3 139)。在肝病组中 ,重型肝炎、肝硬化、肝癌患者的TTVDNA阳性率分别为 35 .71% (5 14 ) ,14 .15 % (15 10 6 )和 15 .79(3 19)。测定的 2 82个核苷酸中 ,3株贵州株的同源性均高于 99% ,与日本株N2 2相比 ,同源性都为 98%。结论 贵州存在TTV感染 ,血透患者中有较高的TTV感染率 ,TTV可能是重型肝炎的病原因子 ,3株贵州株可能为同一基因型。
Objective To understand the status of TTV infection in Guizhou and to analyze the genetic characteristics of TTV Guizhou strain. Methods Two pairs of oligonucleotide primers were designed based on the published sequence of the first reading region of TTV. TTV nucleic acid (TTTVNA) in serum of different populations in Guizhou province was detected by nested PCR. Three TTVDNA Positive serum PCR products were sequenced to determine the nucleotide sequence. Results The positive rates of TTVDNA in serum of 62 normal controls, 37 volunteer blood donors, 50 hemodialysis patients, 107 intravenous drug addicts and 139 patients with liver disease were respectively 6.45% (462), 8.1% (377), 26.0% (1350), 25.3% (2 7 10 7) and 16.55% (2 3 139). In the liver disease group, the positive rates of TTVDNA in patients with severe hepatitis, cirrhosis and liver cancer were 35.71% (5 14), 14.15% (15 10 6) and 15 79 (3 19) respectively. Among the 2 82 nucleotides tested, the homology of three Guizhou strains was higher than 99%, and the homology was 98% compared with that of Japanese strain N2 2. Conclusion TTV infection is present in Guizhou, and the infection rate of TTV in hemodialysis patients is high. TTV may be the pathogenic factor of severe hepatitis, and the three Guizhou strains may be the same genotype.