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本文介绍一种简便可靠单亲无核的细胞融合技术:其融合率和杂交细胞成活和纯度均达实验要求。这种杂交细胞能在体外试管中繁殖和传代(BWXR巳传40几代,而且部分细胞仍能连续表达合成基因产物(血红蛋白),为基团表达调控和诱导肿瘤细胞向正常方向分化的研究提供一种有效的新方法技术,文中对网质红细胞诱发率及细胞融合等进行讨论。
This article describes a simple and reliable single-parent nuclear-free cell fusion technology: the fusion rate and hybrid cell survival and purity reached the experimental requirements. Such hybrid cells can be propagated and passaged in in vitro tubes (BWXR has been passaged for 40 generations, and some cells continue to express synthetic gene products (hemoglobin) for the study of group regulation of expression and induction of tumor cell differentiation in the normal direction An effective new method and technique, the paper discusses the inducing rate of reticulocyte and cell fusion.