论文部分内容阅读
目的观察输注CD4~+CD25~+调节性T细胞对实验性自身免疫性脑脊髓膜炎(EAE)模型大鼠后脑和脊髓组织的保护作用及相关机制。方法取SD大鼠30只,随机均分为空白对照组、模型组(实验性自身免疫性脑脊髓炎模型)和实验组(CD4~+CD25~+调节性T细胞输注),对EAE大鼠进行神经功能评分及体质量测量,通过病理学HE染色和免疫组化观察脑和脊髓炎症浸润,实时荧光定量PCR(qPCR)检测鼠脑和脊髓IFN-γ、IL-17、IL-23和Foxp3 m RNA水平表达。结果实验组SD大鼠神经功能评分明显改善,体质量明显增加;与模型组比较,实验组神经症状和病理改变减轻,鼠脑和脊髓IFN-γ、IL-17、IL-23 mRNA表达下降,而Foxp3 mRNA表达增加(P<0.05)。结论 CD4~+CD25~+调节性T细胞通过调节免疫系统的脑和脊髓IFN-γ、IL-17、IL-23和Foxp3 mRNA水平的表达对EAE起保护作用。
Objective To observe the protective effect of CD4 ~ + CD25 ~ + regulatory T cells on the posterior cerebral and spinal cord tissues in experimental autoimmune meiomyelitis (EAE) rats and its related mechanisms. Methods Thirty SD rats were randomly divided into blank control group, model group (experimental autoimmune encephalomyelitis model) and experimental group (CD4 + CD25 + regulatory T cell infusion) The neurological function scores and body weight were measured. Pathological HE staining and immunohistochemistry were used to observe inflammatory infiltration of brain and spinal cord. Real-time quantitative PCR (qPCR) was used to detect the expression of IFN-γ, IL-17 and IL-23 Foxp3 m RNA level expression. Results Compared with the model group, the neurological symptoms and pathological changes in the experimental group were alleviated, the expression of IFN-γ, IL-17 and IL-23 mRNA in the brain and spinal cord decreased, While Foxp3 mRNA expression increased (P <0.05). Conclusion CD4 ~ + CD25 ~ + regulatory T cells play a protective role in EAE by regulating the expression of IFN-γ, IL-17, IL-23 and Foxp3 mRNA in the brain and spinal cord of immune system.