论文部分内容阅读
目的了解驻湖北孝感某部营区蜱虫及蜱媒病原体感染现状,为防治蜱媒病对人群健康危害提供科学依据。方法 2012年对某营区的仓库及训练场开展蜱虫调查,采集营区警犬饲养员及离营区20 km医院发热待查患者血样、警卫犬体表及营区草地上的蜱虫,分别提取其基因组DNA,PCR方法检测分析测定病原体基因分型。结果累计收集患者血110份,将血样混合分组,共7组;警犬饲养员血1份。患者血样检出巴尔通体和肺炎军团菌分别为3组和1组,最大似然估计(maximum likelihood estinate,MLE)感染率分别为27.77%(4/110),8.52‰(1/110);警犬饲养员血液检测到巴尔通体。从警犬身上、营区草地上分别采集蜱虫6只、20只。警卫犬体表蜱虫和营区草地蜱虫均检测到巴尔通体和立克次体。营区警犬饲养员及医院发热待查患者血样与营区警犬体表蜱虫检测到的巴尔通体基因型不同,分别为牛巴尔通体(B.bovis USAMRIID-000002),杆菌巴尔通体(B.birtlesii USAMRIID-000020),伊莉萨白巴尔通体(B.elizabethae USAMRIID-000008 or B.grahamii USAMRIID-000026),巴尔通体变形菌(B.grahamii USAMRIID-000026);而警犬体表蜱虫携带的为巴尔通伯格霍夫亚种(Bartonella vinsonii subsp.berkhoffii)基因型Ⅲ。不同来源的样本检测的巴尔通体基因型不同。结论该调查点蜱虫易见,蜱媒病原体感染率高,应采取蜱虫防制措施。
Objective To understand the status of tick and tick-borne pathogens in a barrack area in Xiaogan, Hubei Province, and to provide a scientific basis for the prevention and control of tick-borne diseases. Methods In 2012, a tick investigation was conducted on the warehouse and training ground of a camp area. Blood samples of the 20-km hospital fever-guarding battalion and guard tick canine body and ticks on the lawn grass area were collected from the canine breeder in the camp area and genomic DNA , PCR method of detection and analysis of pathogen genotyping. Results A total of 110 blood samples were collected and the blood samples were divided into 7 groups. The prevalences of Bartonella and Legionella pneumophila in blood samples from patients were 3 and 1, respectively. The maximum likelihood of infection (MLE) infection rates were 27.77% (4/110) and 8.52 ‰ (1/110), respectively. Bartender blood Barton body detected. From the police dog body, batches were collected on the meadow grass tick 6, 20. Bartonella and rickettsia were detected in guard dog ticks and camp ticks. Camp beetle breeders and hospital patients with fever to be investigated blood samples and Camp beetle surface tick ticks detected Bartonella genotype different, respectively, bovine Bartonella (B.bovis USAMRIID-000002), Bacillus Barton (B.birtlesii USAMRIID- 000020), B. elizabethae USAMRIID-000008 or B. grahamii USAMRIID-000026, B. grahamii USAMRIID-000026; and police tick tick tick carrying Baltotor Bartonella vinsonii subsp. Berkhoffii genotype Ⅲ. Bartonella genotypes were different for samples from different sources. Conclusion It is easy to see ticks in this investigation point. The infection rate of tick pathogens is high, and tick control measures should be taken.