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用碱性髓鞘蛋白加福氏完全佐剂免疫ＳＪＬ／Ｊ小鼠，建立实验性变态反应性脑脊髓炎动物模型。取发病动物淋巴结作细胞培养，并用碱性髓鞘蛋白激活培养３周。其间２次经Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ分离纯化激活的细胞，并加Ｘ线照射的脾细胞，应用３Ｈ－ＴｄＲ掺入法测定细胞对碱性髓鞘蛋白的反应状况。细胞经ＰＫＨ２－ＧＬ标记及ＰＥ－抗ＣＤ４荧光染色，流式细胞仪分析细胞阳性情况。ＰＫＨ２－ＧＬ标记的细胞静脉注入同品系健康小鼠，约１０天后，动物发生典型的实验性变态反应性脑脊髓炎症状。随之分离发病动物的中枢神经系统和淋巴结淋巴细胞，发现于中枢神经系统中ＰＫＨ２－ＧＬ标记细胞中４５％为ＣＤ４＋细胞，其中９０％以上细胞为激活的母细胞。而在淋巴结中ＣＤ４＋细胞低于４％，母细胞少于１０％，结果说明对碱性髓鞘蛋白反应的ＣＤ４＋细胞，可选择性地进入中枢神经系统并介导实验性变态反应性脑脊髓炎的免疫病理反应。
SJL / J mice were immunized with basic myelin plus carbofuran complete adjuvant to establish an animal model of experimental allergic encephalomyelitis. Lymph nodes were collected for cell culture and activated with alkaline myelin protein for 3 weeks. In the meantime, activated cells were isolated and purified twice by Ficoll-Hypaque, and splenocytes irradiated with X-rays were also measured. 3H-TdR incorporation assay was used to determine the response of cells to basic myelin protein. The cells were stained with PKH2-GL and PE-anti-CD4, and analyzed by flow cytometry. PKH2-GL labeled cells were intravenously injected into healthy, homogenous mice and symptoms of typical experimental allergic encephalomyelitis occurred in animals about 10 days later. As a result, 45% of PKH2-GL-labeled cells in the central nervous system were CD4 + cells, and more than 90% of them were activated blasts. While less than 4% of CD4 + cells and less than 10% of blasts in lymph nodes indicate that CD4 + cells that respond to basic myelin can selectively enter the central nervous system and mediate experimental allergic encephalomyelitis The immune pathological response.