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通过花粉管通道技术将慈姑蛋白酶抑制剂API(ArrowheadProteinaseInhibitor)基因转入棉花 ,获得了转基因植株。经对棉铃虫抗虫性鉴定和PCR、RT PCR、Southernblotting分析 ,证明获得了表达API基因的抗虫棉
Transgenic plants were obtained by transferring Arrowhead Proteinase Inhibitor API into cotton through pollen tube pathway. Through the identification of insect-resistance of cotton bollworm and PCR, RT PCR and Southern blotting analysis, it was proved that the insect-resistant cotton expressing API gene