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目的探讨双酚A对MCF-7细胞内整体DNA甲基化水平及甲基化转移酶的影响。方法分别将浓度为10-6mol/L、10-7mol/L的双酚A作用于MCF-7细胞72 h后,免疫荧光法测5-甲基胞嘧啶的含量,RT-PCR法测DNMT1、DNMT3A、DNMT3B mRNA的表达。结果双酚A(10-6mol/L、10-7mol/L)作用72 h后,与溶剂对照组(0.1%DMSO)相比,5-甲基胞嘧啶的含量下降,差异有统计学意义(P<0.05);RT-PCR结果表明浓度为10-6mol/L双酚A处理组的DNMT1的mRNA的表达下降,10-6mol/L、10-7mol/L双酚A组的DNMT3A和DNMT3B的mRNA的表达均增加,差异有统计学意义(P<0.05)。结论双酚A能够引起MCF-7细胞整体DNA甲基化水平下调,其机制可能是通过改变甲基化转移酶的表达来实现。
Objective To investigate the effects of bisphenol A on global DNA methylation and methylated transferase activity in MCF-7 cells. Methods The concentration of 10-6mol / L, 10-7mol / L of bisphenol A in MCF-7 cells after 72 h, immunofluorescence assay 5-methylcytosine content, RT-PCR DNMT1, DNMT3A, DNMT3B mRNA expression. Results Compared with the solvent control group (0.1% DMSO), the content of 5-methylcytosine decreased after 72 h of bisphenol A (10-6 mol / L, 10-7 mol / L), the difference was statistically significant RT-PCR results showed that the mRNA expression of DNMT1 in the group treated with 10-6mol / L bisphenol A decreased, whereas in DNMT3A and DNMT3B treated with 10-6mol / L and 10-7mol / L bisphenol A mRNA expression increased, the difference was statistically significant (P <0.05). Conclusion Bisphenol A can cause the overall DNA methylation level of MCF-7 cells to be down-regulated. The mechanism may be through changing the expression of methylated transferase.