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分别利用三交组合DH8×登海40和DH86×沈137创建F1代DH群体A和群体B,比较2个不同遗传背景下DH群体子粒中锌及铁铜锰含量的变化,并对2个供试群体2年间的试验结果进行了QTL分析。发现玉米子粒中锌及铁铜锰的含量在不同个体间、不同年份间受环境影响比较大,且表现不稳定;群体内呈现连续性数量性状变化,服从于正态分布。对群体A2年子粒中锌及铁铜锰含量进行QTL定位,结果2007年检测到了5个与这些性状有关的QTL,可解释的遗传变异范围为9.41%~43.67%;2008年检测到9个QTL,可解释的遗传变异范围为11.21%~42.96%。2年间末检测到相同的QTL位点。对群体B进行QTL定位,2年间检测到18个QTL位点,分布于除第5染色体以外的其余9条染色体上。其中,2007年检测到12个QTL,2008年获得6个QTL,相同的QTL为2个,控制锌含量的1个位点位于第3染色体的p-umc1399-p-bnlg1605区段,控制铜含量的1个位点位于第2染色体的bnlg1746区段。
The F1 generation of DH population A and population B were created by DH8 × Denghai 40 and DH86 × Shen 137, respectively. The changes of Zn, Fe, Cu and Mn contents in DH population were compared under two different genetic backgrounds. QTL analysis was carried out on the test results of two groups for two years. It was found that the contents of zinc and copper, manganese and iron in maize were in different individuals and in different years affected by the environment, and their performance was not stable. The population showed continuous quantitative traits, which obeyed the normal distribution. QTL mapping was performed on the A2 population of Ca2 + in F2 population in the group A2. As a result, 5 QTLs related to these traits were detected in 2007, and the range of genetic variation was 9.41% ~ 43.67%. Nine QTLs were detected in 2008 The range of genetic variation that can be explained is 11.21% ~ 42.96%. The same QTL locus was detected at the end of two years. For QTL mapping of population B, 18 QTLs were detected in 2 years and distributed on the remaining 9 chromosomes except chromosome 5. Among them, 12 QTLs were detected in 2007, 6 QTLs in 2008 and 2 identical QTLs. One locus controlling zinc content was located in the p-umc1399-p-bnlg1605 region on chromosome 3 to control copper content One locus is located on the bnlg1746 segment of chromosome 2.