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目的:探索从骨纤维异常增殖症(FD)病变组织中分离、培养骨髓间充质干细胞(MSC)的有效方法,为研究骨纤维异常增殖症的病因及发病机制奠定基础。方法:取FD患者新鲜病变组织,将其剪碎后冲洗,获得骨髓间充质干细胞,进行培养,观察细胞形态,绘制细胞生长曲线,检测其细胞表面标志物及向成骨细胞的诱导分化能力。结果:培养的骨髓干细胞随传代次数增多,细胞形态趋向长梭形,99%的细胞表达CD44和HLA-ABC,不表达CD34;可见诱导后的MSC碱性磷酸酶染色阳性,并形成钙结节。结论:在新鲜FD骨标本中可以成功提取MSC,其在体外具有诱导成骨的能力,获取到的MSC可以满足后续实验的要求。
Objective: To explore an effective method for the isolation and culture of bone marrow mesenchymal stem cells (MSC) from the fibrous dysplasia of bone (FD), and to lay a foundation for the study of the etiology and pathogenesis of bone dysplasia. Methods: Fresh diseased tissues of FD patients were obtained. After being cut and rinsed, the bone marrow mesenchymal stem cells were obtained and cultured. The morphology of the cells was observed and the cell growth curve was drawn. The cell surface markers and differentiation of osteoblasts were assayed . Results: The number of bone marrow stem cells increased with the number of passages and the morphology of the cells became fusiform. 99% of the cells expressed CD44 and HLA-ABC, but did not express CD34. Positive MSC alkaline phosphatase staining and calcium formation . CONCLUSION: MSCs can be successfully extracted from fresh FD bone specimens and have the ability to induce osteogenesis in vitro. The acquired MSCs can meet the requirements of subsequent experiments.