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本文用正交试验方法选择血样的水解条件。在样品中加入浓HCl。溶液HCl浓度为1.0mol/L。水解45分钟,中和后萃取TNT及两种代谢产物4A和2A。该条件为最佳水解条件。萃取剂为乙醚,提取比为1:1,溶液PH=8。振荡萃取2分钟,萃取率90.4～99.0%,平均94.6%。用HPLC法使用5μm反相C_(18)离于对色谱柱,甲醇/水系统,分离TNT、4A和2A分离效果良好。本方法已用于133名接触TNT工人和18名非接触工人血样测定,能满足现场测定需要。
In this paper, the orthogonal test method to select the hydrolysis conditions of blood samples. Add concentrated HCl to the sample. The solution HCl concentration was 1.0 mol / L. After 45 minutes of hydrolysis, TNT and two metabolites 4A and 2A were extracted after neutralization. The conditions for the optimal hydrolysis conditions. Extractant is ether, the extraction ratio is 1: 1, the solution PH = 8. Oscillatory extraction 2 minutes, the extraction rate of 90.4 ~ 99.0%, an average of 94.6%. The separation of TNT, 4A and 2A by 5μL reversed-phase C_ (18) by HPLC on column, methanol / water system was good. This method has been used in 133 TNT workers exposed and 18 non-contact workers blood samples, to meet the field measurement needs.