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目的:探讨自分泌运动因子(AMF)在人肝细胞癌侵袭和转移中的作用。方法:人肝细胞系LO2和人肝细胞癌细胞株MHCC97-H作为实验材料,检测二者AMF的表达水平;设计并合成针对AMF基因序列的双链小干扰RNA转染高转移性人肝癌细胞株MHCC97-H,Western blot检测AMF基因的蛋白的表达水平;通过MTT实验检测转染后细胞的增殖力;通过体外Transwell小室对比沉默AMF基因前后的肝癌细胞的迁移力和侵袭力;最后用细胞悬液皮下接种小鼠,观察沉默AMF基因前后肝细胞的成瘤能力。结果:AMF在MHCC97-H的表达量较高;将双链小干扰RNA转入MHCC97-H后,AMF的表达显著降低(P<0.05);沉默AMF基因序列后,MHCC97-H的增殖力、迁移力和侵袭力均有明显下降(P<0.05);用细胞悬液皮下接种小鼠沉默AMF基因的MHCC97-H形成的肿瘤体积小于对照组(P<0.05)。结论:AMF基因可调节肝癌细胞的迁移和侵袭。
Objective: To investigate the role of autotaxin (AMF) in the invasion and metastasis of human hepatocellular carcinoma. METHODS: Human hepatocyte cell line LO2 and human hepatocellular carcinoma cell line MHCC97-H were used as experimental materials to detect the expression level of AMF. The double-stranded small interfering RNA targeting AMF gene was transfected into highly metastatic human hepatoma cells Strain MHCC97-H and Western blot were used to detect the expression of AMF gene. MTT assay was used to detect the proliferation of transfected cells. Migration and invasion ability of AMF gene in vitro and in vivo were analyzed by Transwell assay. The mice were inoculated subcutaneously with the suspension to observe the tumorigenicity of the hepatocytes before and after silencing the AMF gene. Results: The expression of AMF was higher in MHCC97-H. The expression of AMF was significantly lower in MHCC97-H transfected with double-stranded small interfering RNA (P <0.05). After AMF gene silencing, (P <0.05). The volume of tumor formed by MHCC97-H silencing AMF gene in mice subcutaneously inoculated with cell suspension was smaller than that of control group (P <0.05). Conclusion: AMF gene can regulate the migration and invasion of hepatocellular carcinoma cells.