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目的研究毒死蜱对肺组织的氧化损伤和维生素E的抗氧化作用。方法昆明小鼠随机分为5组,包括1个阴性对照组(花生油)、3个毒死蜱(低、中、高剂量)染毒组(3、6和12 mg/kg)和1个高剂量毒死蜱(12 mg/kg)+维生素E(100mg/kg)组,连续灌胃7 d,测定肺组织匀浆活性氧(ROS)、还原型谷胱甘肽(GSH)和肺组织细胞DNA-蛋白质交联(DPC)系数;并观察病理损伤。结果与对照组比较,中、高剂量组ROS含量、DPC系数升高,GSH含量降低(P<0.05,P<0.01)。病理学观察可见中、高剂量组肺泡隔血管扩张充血,肺泡壁增厚,肺泡腔内有红细胞聚集。与高剂量组相比较,高剂量毒死蜱+VE组肺组织的ROS含量和DPC系数均有下降,GSH含量上升(P<0.05)。结论较高剂量(6、12 mg/kg)的毒死蜱能造成小鼠肺组织的氧化损伤和病理损伤,且可以被维生素E的抗氧化作用所拮抗。
OBJECTIVE To study the oxidative damage of lung tissue and the anti-oxidation of vitamin E by chlorpyrifos. Methods Kunming mice were randomly divided into 5 groups, including 1 negative control group (peanut oil), 3 chlorpyrifos (low, medium and high dose) exposure groups (3, 6 and 12 mg / kg) and 1 high dose of chlorpyrifos (12 mg / kg) and vitamin E (100 mg / kg) for 7 days. The levels of reactive oxygen species (ROS), reduced glutathione (GSH) and DNA- (DPC) coefficient; and observe the pathological injury. Results Compared with the control group, the content of ROS and DPC of middle and high dose groups increased and the content of GSH decreased (P <0.05, P <0.01). Pathological observation shows that medium and high doses of alveolar septal dilatation and congestion, alveolar wall thickening, alveolar cavity erythrocyte aggregation. Compared with the high dose group, the content of ROS and DPC in lungs of high dose chlorpyrifos + VE group decreased and the content of GSH increased (P <0.05). Conclusion Chlorpyrifos at higher doses (6, 12 mg / kg) can cause oxidative and pathological damage in the lung tissue of mice and can be antagonized by the antioxidant effect of vitamin E.