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在研究人及某些脊椎动物视神经筛板的发育及结构过程中,我们将Luna弹性纤维染色法加以改良,后发现新方法能较清晰地显示弹性纤维的组织分布情况。现介绍如下。1 材料和方法标本用10%中性缓冲甲醛溶液或2.5%～5%戊二醛磷酸缓冲固定液(pH7.4)固定。石蜡包埋、切片厚5μm。染液配方:
In the study and the development of vertebrate optic nerve plate and its structure, we modified Luna elastic fiber method and found that the new method can clearly show the distribution of elastic fiber tissue. Now introduced as follows. 1 Materials and methods Specimens were fixed with 10% neutral buffered formaldehyde solution or 2.5% ~ 5% glutaraldehyde phosphate buffered solution (pH7.4). Paraffin embedded, section thickness 5μm. Dye formula