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目的观察沼气池对血吸虫卵杀灭作用,沼气液对钉螺、尾蚴杀灭作用,以及沼气液对农业增产的综合效益。方法将含活血吸虫卵的阳性人粪以模拟自然排粪状态投放到沼气池中,用260目尼龙袋装4包有血吸虫卵(每包40 g)的人粪投放到发酵池中浸泡,7、15、30、60 d分别取出1包粪样进行孵化,Kato-Katz检查虫卵的死活;进行沼气液杀灭钉螺、尾蚴实验;沼气液对小麦施肥效果观察。结果以260目尼龙绢袋包裹方式投放后,15 d后血吸虫孵化计数为零,沼气液杀灭钉螺室内为6.7%,现场为8.3%,杀灭尾蚴实验20 min后原液死亡率94.3%,50%、25%浓度死亡率均为100%,对照组无死亡。施用沼液组小麦产量为56 kg,农家肥组产量57.5 kg,化肥组产量60 kg,对照组产量35.5 kg。结论沼液对血吸虫卵有明显的杀灭作用;对钉螺杀灭效果不明显;对尾蚴有显著的杀灭效果,小麦增产方面沼液组与其他组不明显。
Objective To observe the killing effect of biogas digester on schistosome eggs, the killing effect of biogas slurry on snail and cercariae, and the comprehensive benefits of biogas slurry on agricultural production. Methods A positive human excrement containing live schistosome eggs was injected into the biogas digester in a simulated natural decontamination condition. Four human skulls of schistosome eggs (40 g per pack) One package of fecal samples were taken out for incubation at 15, 30 and 60 days respectively. Kato-Katz was used to check the death and survival of the eggs. Biogas slurry was used to kill snails and cercariae. Biogas slurry was used to fertilize wheat. The results showed that the schistosome hatch count was zero after 15 days, and the kill rate of the schistosomula was 6.7% in the biogas slurry and 8.3% in the field. After 20 min of killing the cercariae, the mortality of the schistosoma liquid was 94.3% and 50 %, 25% mortality were 100%, the control group no death. In the biogas slurry group, the yield of wheat was 56 kg, that of farmyard manure was 57.5 kg, that of chemical fertilizer was 60 kg, and that of the control was 35.5 kg. Conclusion The biogas slurry could kill schistosome eggs obviously, kill the snail effectively and kill the cercariae obviously. The biogas production of the wheat was not obvious with other groups.