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用羟胺将光系统Ⅱ中的锰去除后，可导致光系统Ⅱ的蛋白二级结构组成发生变化，表现在α－螺旋成分的减少及转角和折叠成分的增加。将锰重组进光系统Ⅱ后，蛋白的二级结构不能恢复，并导致转角和折叠成分向无规卷曲转化。去锰后的光系统Ⅱ所发生的由供体侧电子传递受阻引起的光抑制，会在短时间内引起蛋白的构象变化。但锰重组后其在光抑制下的构象变化与正常样品相似，说明此时供体侧的电子传递得到部分恢复，光抑制部分地转移到受体侧。
The removal of manganese from photosystem II by hydroxylamine resulted in the change of the secondary structure of the protein of photosystem Ⅱ, which showed the decrease of α-helix and the increase of the angle and the folding component. After recombination of manganese into Photosystem II, the secondary structure of the protein can not be recovered and leads to random coil transformation of the corners and folded components. Photo-inhibition by photoreceptor-donor side photon system II, which occurs after the manganese removal, causes a conformational change of the protein in a short period of time. However, its conformational change under photoinhibition was similar to that of normal samples after manganese recombination, indicating that the donor side electron transfer was partly restored and the photoinhibition partially transferred to the receptor side.