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目的制备产朊假丝酵母尿酸酶(uricase,URI)脂质纳米粒,并分析其药效学特性。方法采用旋转蒸发法制备3批URI脂质纳米粒(lipid nanoparticles containing uricase from Candida utilis,LNURI),检测其包封率、URI活性、酶的最适作用温度和最适作用pH值。采用次黄嘌呤和氧嗪酸钾建立高尿酸大鼠模型,分别于建模后1 h经尾静脉注射LNURI和游离URI,建模后3、5、7、8、12 h,测定大鼠血清中尿酸水平。结果制备的3批LNURI的平均包封率为(64.27±2.26)%;LNURI的最适作用温度为40℃,最适作用pH值为8.0,在同样的作用温度和pH值条件下,LNURI的活性明显高于游离URI;LNURI降低高尿酸模型大鼠血清中尿酸水平的效果较URI更显著。结论成功制备了产朊假丝酵母尿酸酶脂质纳米粒,其可降低高尿酸模型大鼠血清中尿酸水平。
Objective To prepare uricase (uricase, uricase) lipid nanoparticles and analyze its pharmacodynamic properties. Methods Three batches of SODURI (LNURI) were prepared by rotary evaporation method. The entrapment efficiency, URI activity, optimum temperature and optimum pH of enzyme were measured. Hypoxanthine and oteracil potassium were used to establish rat model of hyperuricemia. LNURI and free URI were injected via caudal vein 1 h after modeling, respectively. After 3, 5, 7, 8 and 12 h of modeling, rat serum Uric acid levels. Results The average entrapment efficiency of three LNURI batches was (64.27 ± 2.26)%. The optimum temperature of LNURI was 40 ℃ and the optimum pH was 8.0. Under the same temperature and pH conditions, the LNURI Activity was significantly higher than the free URI; LNURI lower serum uric acid levels in rats with hyperuricemia effect is more significant than the URI. Conclusion The Candida utilis uricase lipid nanoparticles were successfully prepared, which can reduce the level of uric acid in the serum of rats with hyperuricemia.