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抗菌剂α-炔丙基-γ-亚甲基-γ-丁内酯是以天然抗菌物质原白头翁素为基础,利用仿生技术合成出的新型抗菌剂。通过悬液杀菌法进行最小抑菌浓度测定、悬液定量法及活体菌落计数法进行最小杀菌浓度测定等生化试验对其抗菌效果进行了检验;通过细胞壁结构的完整性实验及透射电镜观察等理化实验对其抗菌机理做初步研究,为其应用于实践提供依据。抗菌剂α-炔丙基-γ-亚甲基-γ-丁内酯对大肠杆菌、金黄色葡萄球菌、白色念珠菌及马拉色菌均具有良好的抑菌作用,对马拉色菌的抑菌作用尤为突出,并对其具有良好的杀菌作用。其对马拉色菌的最小抑菌浓度为0.5mg/ml,当其浓度在10mg/ml时,对马拉色菌具有瞬杀作用(作用1min对球形马拉色菌的杀灭率可达到99.99%)。通过胞壁完整性实验以及电镜观察实验表明该抗菌剂的抑菌机制并不是完全破坏胞壁结构,但是对细胞壁有一定影响。
Antiseptics α-propargyl-γ-methylene-γ-butyrolactone is a new type of antibacterial agent synthesized on the basis of the original anti-bacterial substance of Pulsatilin using biomimetic technology. The antibacterial effect was tested by the suspension bactericidal method for the determination of the minimum inhibitory concentration, the suspension quantitative assay and the minimum bactericidal concentration determination by the live colony counting method. The cell wall structure integrity test and transmission electron microscopy Experiments on its antimicrobial mechanism to do a preliminary study, to provide the basis for its application in practice. Antibacterial agents α-propargyl-γ-methylene-γ-butyrolactone Escherichia coli, Staphylococcus aureus, Candida albicans and Malassezia have a good antibacterial effect on Malassezia Antibacterial effect is particularly prominent, and its good bactericidal effect. The minimum inhibitory concentration against Malassezia was 0.5mg / ml. When its concentration was 10mg / ml, it had an instant kill effect on Malassezia (the killing rate of M. globosa could reach 99.99%). The experiments of cell wall integrity and electron microscopy showed that the bacteriostatic mechanism of the antibacterial agent did not completely destroy the cell wall structure but had some influence on the cell wall.