OBJECTIVE:To investigate the inhibitory effect of Jinghua Weikang capsule (JWC) on gastric inflammation induced by Helicobacter pylori (H.pylori) via the nuclear factor-kappa B (NF-κB) signaling pathway in Kunming mice.METHODS:We investigated the anti-inflammation potential of JWC extract in vivo in a H.pylori-induced gastritis mouse model.The expression of inflammation-related molecules was evaluated by West blotting,and the concentrations of in vivo inflammatory markers were measured by enzyme-linked immunosorbent assay.Inflammatory cell infiltration was evaluated by histopathological examination,and mRNA levels of related genes were evaluated by quantitative reverse transcription polymerase chain reaction.RESULTS:JWC had a dose-dependent protective effect against H.pylori-induced gastritis by protecting gastric epithelial cells and inhibiting inflammatory cell infiltration.Mechanistically,JWC decreased the protein levels of phosphorylated IκBa and NF-κB p65,mRNA levels of NF-κB pathway molecules,and plasma levels of tumor necrosis factor-α and interleukin 1 beta.CONCLUSION:An important finding of our study is that JWC attenuated gastrointestinal inflammation and ulceration and exerted a protective effect against gastric injury via inhibition of inflammation reactions and regulating the canonical NF-κB signaling pathway in vivo.