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观察酒精对大鼠部分肝切除后残肝再生的影响以及中药丹参对酒精性肝病大鼠残肝再生的效果，以期初步探讨酒精性肝病的发生机制，同时为丹参在酒精性肝病防治方面的应用提供理论依据。方法：实验分３组①对照组１０只：每日自由饮食；②酒精组１０只：每天定量灌胃酒精外，鼠自由饮食；③丹参组１０只：在灌胃酒精（方法同酒精组）４周后，同时每日腹腔注射丹参注射液，１２０ｇ／ｋｇ／ｄ。各组大鼠至６周末行部分肝叶切除术，术后２４小时将大鼠处死，留取肝组织制备肝细胞悬液，行流式细胞术分析，绘制出ＤＮＡ直方图以增殖指数（ＰＩ）和Ｓ期比例（ＳＰＦ）表示细胞增殖活性。另取肝组织以１０％福尔马林固定，ＨＥ染色。结果：①肝细胞增殖周期时相分布情况：酒精组ＤＮＡ直方力冯Ｇ０／Ｇ１期细胞为主峰，但其峰值与对照组相比明显增加，Ｓ期峰抬高不明显，Ｇ２Ｍ峰较低平。丹参组ＤＮＡ直方图Ｇ０／Ｇ１峰明显低于酒精组，Ｓ期抬高及Ｇ２Ｍ峰较酒精组明显；②ＰＩ和ＳＰＦ对比：酒精组ＰＩ和ＳＰＦ值明显低于对照组及丹参组（Ｐ＜００５及＜００１），丹参组ＰＩ及ＳＰＦ值虽低于对照组，但两组比较差异无显著性（Ｐ＞００５）；③病理学：酒精组大鼠肝细胞明显肿胀，胞浆内可见大量脂滴?
To observe the effect of alcohol on residual liver regeneration after partial hepatectomy in rats and the effect of traditional Chinese medicine Danshen on residual liver regeneration in rats with alcoholic liver disease so as to investigate the mechanism of alcoholic liver disease and to apply Salvia miltiorrhiza in the prevention and treatment of alcoholic liver disease Provide a theoretical basis. Methods: The experiment was divided into 3 groups ① control group of 10: free diet; ② alcohol group 10: daily quantitative oral administration of alcohol, rats free diet; ③ Salvia group 10: in the intragastric administration of alcohol (method with alcohol group) Four weeks later, intraperitoneal injection of Danshen injection at the same time, 120g / kg / d. The rats in each group were subjected to partial hepatectomy until the 6th weekend, and the rats were sacrificed 24 hours after the operation. Liver tissues were harvested to prepare a suspension of hepatocytes. Flow cytometry analysis was performed to draw the DNA histogram with the proliferation index ) And S phase ratio (SPF) represent cell proliferative activity. Another liver tissue fixed in 10% formalin, HE staining. Results: ①The phase distribution of hepatocyte proliferation cycle: The peak of G0 / G1 phase in alcohol group was the main peak, but the peak value was significantly higher than that of the control group, the elevation of S phase peak was not obvious, the G2M peak was lower . Salvia miltiorrhiza DNA histogram G0 / G1 peak was significantly lower than the alcohol group, S-phase elevation and G2M peak than the alcohol group significantly; ② PI and SPF comparison: alcohol group PI and SPF values were significantly lower than the control group and Salvia group (P <0 ⑤05 and <001). The PI and SPF values in Salvia miltiorrhiza group were lower than those in the control group, but there was no significant difference between the two groups (P> 0.05). ③ Pathology: The hepatocytes in the alcohol group were obviously swollen, Cytoplasm can be seen a large number of lipid droplets?